[白细胞介素-4、γ-干扰素基因甲基化在新疆维吾尔族、汉族变应性鼻炎表观遗传学中的比较研究]

[Comparative study of IL-4, IFN-gamma gene methylation for the epigenetics of allergic rhinitis in Xinjiang Uygur, Han people].

作者信息

Lou Zhongzhang, Wang Huiwu, Yang Qing, Jiang Xiaofang, Zhang Qingquan, Mu Ni Re, Mu He Ta Er, Gao Li

机构信息

Department of Otorhinolaryngology, First Affiliated Hospital of Xinjiang Medical University, Urumqi 230001, China.

出版信息

Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2012 Sep;26(18):795-7.

PMID:23259293

Abstract

OBJECTIVE

To investigate the differences of IL-4, IFN-gamma gene promoter methylation of allergic rhinitis patients between Uygur and Han people of Xinjiang.

METHOD

Methylation-specific PCR (MSP) detected IL-4, IFN-gamma gene methylation of each of 50 patients with allergic rhinitis in Uygur and Han.

RESULT

Complete IL-4 gene promoter methylation rate was 44% (22/50) and 48% (24/50) in Uygur and Han AR patients, un-methylation was 26% (13/50) and 22% (11/50), coexistence rate of methylation and un-methylation was 30% (15/50) and 30% (15/50). Complete IFN-gamma gene promoter methylation rate was 12% (6/50) and 16% (8/50) in Uygur and Han AR patients, un-methylation was 8% (4/50) and 10% (5/50), coexistence rate of methylation and unmethylated was 80% (40/50) and 74% (37/50). Distribution of IL-4 gene methylation between Han and Uygur AR patients had no statistically significant (P > 0.05). Distribution of IFN-gamma gene methylation between Han and Uygur AR patients had no statistically significant (P > 0.05).

CONCLUSION

There is no difference of IL-4, IFN-gamma gene methylation in patients between the Han and Uygur.

摘要

目的

探讨新疆维吾尔族和汉族变应性鼻炎患者白细胞介素-4（IL-4）、γ干扰素（IFN-γ）基因启动子甲基化的差异。

方法

采用甲基化特异性PCR（MSP）法检测50例维吾尔族和汉族变应性鼻炎患者IL-4、IFN-γ基因的甲基化情况。

结果

维吾尔族和汉族变应性鼻炎患者中IL-4基因启动子完全甲基化率分别为44%（22/50）和48%（24/50），未甲基化率分别为26%（13/50）和22%（11/50），甲基化与未甲基化共存率分别为30%（15/50）和30%（15/50）。维吾尔族和汉族变应性鼻炎患者中IFN-γ基因启动子完全甲基化率分别为12%（6/50）和16%（8/50），未甲基化率分别为8%（4/50）和10%（5/50），甲基化与未甲基化共存率分别为80%（40/50）和74%（37/50）。汉族和维吾尔族变应性鼻炎患者IL-4基因甲基化分布差异无统计学意义（P>0.05）。汉族和维吾尔族变应性鼻炎患者IFN-γ基因甲基化分布差异无统计学意义（P>0.05）。