Institute of Nephrology, Graduate School of Medical and Dental Sciences, Niigata, Japan.
J Proteome Res. 2013 Jan 4;12(1):58-61. doi: 10.1021/pr300844p. Epub 2012 Dec 21.
This article introduces how the human chromosome X-centric proteome project is carried out by the Japan Chromosome X Project Consortium. The inactivation of one of two chromosomes in female mammals and accumulation of genes related to neural/immune systems/tumor/testis are characteristic of chromosome X. In this Chromosome X Project, information on proteins translated from genes on chromosome X is collected by both mass spectrometry- and antibody-based proteomics. Information on the following resources is also provided: antibodies to proteins translated and full-length cDNAs transcripted from the chromosome X genes for recombinant proteins. The consortium aims to provide the following tools to search useful antibodies in the literature (Antibody Ranker), to find gene expression sites in microarray databases (Transcript Localizer) and to do advanced MRM analysis (information-based MRM).
本文介绍了日本染色体 X 计划联盟如何开展人类染色体 X 中心蛋白质组项目。雌性哺乳动物中两条染色体之一的失活以及与神经/免疫系统/肿瘤/睾丸相关的基因积累是染色体 X 的特征。在这个染色体 X 项目中，通过质谱和抗体为基础的蛋白质组学收集了从染色体 X 上的基因翻译而来的蛋白质的信息。还提供了以下资源的信息：针对染色体 X 基因翻译的蛋白质和全长 cDNA 转录的重组蛋白的抗体。该联盟的目标是提供以下工具，以在文献中搜索有用的抗体（抗体排名），在微阵列数据库中找到基因表达位点（转录定位器），并进行高级 MRM 分析（基于信息的 MRM）。
