Pedersoli Wellington Ramos, de Paula Renato Graciano, Antoniêto Amanda Cristina Campos, Carraro Cláudia Batista, Taveira Iasmin Cartaxo, Maués David Batista, Martins Maíra Pompeu, Ribeiro Liliane Fraga Costa, Damasio André Ricardo de Lima, Silva-Rocha Rafael, Filho Antônio Rossi, Silva Roberto N
Department of Genetics, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, SP, 14049-900, Brazil.
Department of Biochemistry and Immunology, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, SP, 14049-900, Brazil.
Biotechnol Rep (Amst). 2021 Jun 22;31:e00652. doi: 10.1016/j.btre.2021.e00652. eCollection 2021 Sep.
is one of the major producers of holocellulases. It is known that in , protein production patterns can change in a carbon source-dependent manner. Here, we performed a phosphorylome analysis of grown in the presence of sugarcane bagasse and glucose as carbon source. In presence of sugarcane bagasse, a total of 114 phosphorylated proteins were identified. Phosphoserine and phosphothreonine corresponded to 89.6% of the phosphosites and 10.4% were related to phosphotyrosine. Among the identified proteins, 65% were singly phosphorylated, 19% were doubly phosphorylated, 12% were triply phosphorylated, and 4% displayed even higher phosphorylation. Seventy-five kinases were predicted to phosphorylate the sites identified in this work, and the most frequently predicted serine/threonine kinase was PKC1. Among phosphorylated proteins, four glycosyl hydrolases were predicted to be secreted. Interestingly, Cel7A activity, the most secreted protein, was reduced to approximately 60% after dephosphorylation, suggesting that phosphorylation might alter Cel7A structure, substrate affinity, and targeting of the substrate to its carbohydrate-binding domain. These results suggest a novel post-translational regulation of Cel7A.
是全纤维素酶的主要生产者之一。已知在……中，蛋白质生产模式会以碳源依赖的方式发生变化。在此，我们对在甘蔗渣和葡萄糖作为碳源存在的情况下生长的……进行了磷酸化蛋白质组分析。在甘蔗渣存在的情况下，共鉴定出114种磷酸化蛋白质。磷酸丝氨酸和磷酸苏氨酸占磷酸化位点的89.6%，10.4%与磷酸酪氨酸有关。在鉴定出的蛋白质中，65%为单磷酸化，19%为双磷酸化，12%为三磷酸化，4%表现出更高程度的磷酸化。预计有75种激酶会使本研究中鉴定出的位点发生磷酸化，最常预测的丝氨酸/苏氨酸激酶是PKC1。在磷酸化蛋白质中，预计有四种糖基水解酶会被分泌。有趣的是，最主要分泌的蛋白质Cel7A在去磷酸化后活性降低至约60%，这表明磷酸化可能会改变Cel7A的结构、底物亲和力以及底物向其碳水化合物结合结构域的靶向性。这些结果提示了一种关于Cel7A的新型翻译后调控机制。
