Department of Biomedical Engineering, Boston University, 44 Cummington St, Boston, Massachusetts 02215, USA.
ACS Nano. 2013 Feb 26;7(2):1408-14. doi: 10.1021/nn3051677. Epub 2012 Dec 31.
Controlling DNA translocation speed is critically important for nanopore sequencing as free electrophoretic threading is far too rapid to resolve individual bases. A number of promising strategies have been explored in recent years, largely driven by the demands of next-generation sequencing. Engineering DNA-nanopore interactions (known to dominate translocation dynamics) with organic coatings is an attractive method as it does not require sample modification, processive enzymes, or complicated and expensive fabrication steps. In this work, we show for the first time 4-fold tuning of unfolded, single-file translocation time through small, amine-functionalized solid-state nanopores by varying the solution pH in situ. Additionally, we develop a simple analytical model based on electrostatic interactions to explain this effect which will be a useful tool in designing future devices and experiments.
控制 DNA 易位速度对于纳米孔测序至关重要，因为自由电泳穿线速度太快，无法解析单个碱基。近年来，许多有前途的策略已经被探索，主要是受到下一代测序的需求推动。通过有机涂层工程化 DNA-纳米孔相互作用（已知主导易位动力学）是一种有吸引力的方法，因为它不需要样品修饰、连续酶或复杂且昂贵的制造步骤。在这项工作中，我们首次展示了通过原位改变溶液 pH 值，对未折叠、单链易位时间进行 4 倍调节，通过小的、胺功能化的固态纳米孔。此外，我们还开发了一个基于静电相互作用的简单分析模型来解释这种效应，这将是设计未来器件和实验的有用工具。
