利用细胞再循环系统以高细胞密度从木糖连续发酵生产丁醇。
Continuous butanol fermentation from xylose with high cell density by cell recycling system.
机构信息
Laboratory of Microbial Technology, Division of Applied Molecular Microbiology and Biomass Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
出版信息
Bioresour Technol. 2013 Feb;129:360-5. doi: 10.1016/j.biortech.2012.11.066. Epub 2012 Nov 28.
A continuous butanol production system with high-density Clostridium saccharoperbutylacetonicum N1-4 generated by cell recycling was established to examine the characteristics of butanol fermentation from xylose. In continuous culture without cell recycling, cell washout was avoided by maintaining pH>5.6 at a dilution rate of 0.26 h(-1), indicating pH control was critical to this experiment. Subsequently, continuous culture with cell recycling increased cell concentration to 17.4 g L(-1), which increased butanol productivity to 1.20 g L(-1) h(-1) at a dilution rate of 0.26 h(-1) from 0.529 g L(-1) h(-1) without cell recycling. The effect of dilution rates on butanol production was also investigated in continuous culture with cell recycling. Maximum butanol productivity (3.32 g L(-1) h(-1)) was observed at a dilution rate of 0.78 h(-1), approximately 6-fold higher than observed in continuous culture without cell recycling (0.529 g L(-1) h(-1)).
建立了一个连续的丁醇生产系统，该系统使用高密度的细胞循环生成的产丁醇梭菌 N1-4，以研究从木糖发酵生产丁醇的特性。在没有细胞循环的连续培养中，通过维持 pH>5.6 在稀释率为 0.26 h(-1) 避免了细胞洗出，表明 pH 控制对该实验至关重要。随后，通过细胞循环的连续培养将细胞浓度提高到 17.4 g L(-1)，在稀释率为 0.26 h(-1) 时，丁醇生产率从没有细胞循环时的 0.529 g L(-1) h(-1) 增加到 1.20 g L(-1) h(-1)。还研究了细胞循环连续培养中稀释率对丁醇生产的影响。在稀释率为 0.78 h(-1) 时观察到最大丁醇生产率（3.32 g L(-1) h(-1)），约比没有细胞循环的连续培养（0.529 g L(-1) h(-1)）高 6 倍。
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