Laboratory of Microbial Technology, Division of Applied Molecular Microbiology and Biomass Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
Bioresour Technol. 2013 Feb;129:360-5. doi: 10.1016/j.biortech.2012.11.066. Epub 2012 Nov 28.
A continuous butanol production system with high-density Clostridium saccharoperbutylacetonicum N1-4 generated by cell recycling was established to examine the characteristics of butanol fermentation from xylose. In continuous culture without cell recycling, cell washout was avoided by maintaining pH>5.6 at a dilution rate of 0.26 h(-1), indicating pH control was critical to this experiment. Subsequently, continuous culture with cell recycling increased cell concentration to 17.4 g L(-1), which increased butanol productivity to 1.20 g L(-1) h(-1) at a dilution rate of 0.26 h(-1) from 0.529 g L(-1) h(-1) without cell recycling. The effect of dilution rates on butanol production was also investigated in continuous culture with cell recycling. Maximum butanol productivity (3.32 g L(-1) h(-1)) was observed at a dilution rate of 0.78 h(-1), approximately 6-fold higher than observed in continuous culture without cell recycling (0.529 g L(-1) h(-1)).
建立了一个连续的丁醇生产系统，该系统使用高密度的细胞循环生成的产丁醇梭菌 N1-4，以研究从木糖发酵生产丁醇的特性。在没有细胞循环的连续培养中，通过维持 pH>5.6 在稀释率为 0.26 h(-1) 避免了细胞洗出，表明 pH 控制对该实验至关重要。随后，通过细胞循环的连续培养将细胞浓度提高到 17.4 g L(-1)，在稀释率为 0.26 h(-1) 时，丁醇生产率从没有细胞循环时的 0.529 g L(-1) h(-1) 增加到 1.20 g L(-1) h(-1)。还研究了细胞循环连续培养中稀释率对丁醇生产的影响。在稀释率为 0.78 h(-1) 时观察到最大丁醇生产率（3.32 g L(-1) h(-1)），约比没有细胞循环的连续培养（0.529 g L(-1) h(-1)）高 6 倍。
Zotero 插件