Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.
Int J Biol Macromol. 2013 Mar;54:258-63. doi: 10.1016/j.ijbiomac.2012.12.018. Epub 2012 Dec 19.
The molten globule (MG) state is an intermediate which is considered as the third thermodynamic state of protein molecules. In this work the effect of incubating human serum albumin (HSA) at physiological condition in the presence of 3-β-hydroxybutyrate for 7, 14, 21 and 35 days were studied by different techniques such as UV/vis, fluorescence and circular dichroism (CD) spectroscopy, differential scanning calorimetry (DSC) and dynamic light scattering (DLS). In this paper, we introduce the MG state for HSA upon 21 days incubation with 3-β-hydroxybutyrate as a ketone body at physiological condition. The results from the HSA sample incubated for 21 days shows a similar secondary structure by CD, more surface hydrophobicity and a little change on tertiary structure by fluorescence, and a larger size by DLS as compared to the native sample or other incubated samples. These results were also confirmed by calculated parameters and DSC deconvoluted thermograms.
无定形团簇（MG）状态是一种中间状态，被认为是蛋白质分子的第三种热力学状态。在这项工作中，通过不同的技术，如紫外/可见分光光度法、荧光和圆二色性（CD）光谱法、差示扫描量热法（DSC）和动态光散射（DLS），研究了在生理条件下存在 3-β-羟基丁酸的情况下，人血清白蛋白（HSA）孵育 7、14、21 和 35 天的影响。在本文中，我们介绍了在生理条件下用 3-β-羟基丁酸作为酮体孵育 21 天后 HSA 的 MG 状态。与天然样品或其他孵育样品相比，用 CD 显示出相似的二级结构、更高的表面疏水性和荧光稍微改变三级结构、DLS 显示更大的尺寸的 21 天孵育的 HSA 样品。这些结果也通过计算参数和 DSC 解卷积热谱图得到了证实。
