Wei Long-Xiao, Zhou Run-Suo, Xu Hai-Feng, Wang Jun-Yan, Yuan Meng-Hui
Department of Nuclear Medicine, Tangdu Hospital, Fourth Military Medical University, No. 1, Xinsi Rd, Xi'an, 710038, China.
Tumour Biol. 2013 Apr;34(2):941-6. doi: 10.1007/s13277-012-0629-3. Epub 2012 Dec 22.
The aim of this study was to detect FOXC1 expression in human non-small cell lung cancer (NSCLC) and to analyze its association with prognosis of NSCLC patients. Expressional levels of FOXC1 mRNA and protein in 30 cases of NSCLC and corresponding non-tumor tissue samples were examined by quantitative real-time PCR and Western blotting. Immunohistochemistry was performed to detect the expression of FOXC1 in 125 NSCLC tissues. We found that the expression levels of FOXC1 mRNA and protein in NSCLC tissues were significantly higher than those in corresponding non-tumor tissues. High-level FOXC1 expression was correlated with poor tumor differentiation, tumor-node-metastasis stage, and lymph node metastasis. Patients with high expression levels of FOXC1 showed lower overall survival rate than those with low expression levels. Multivariate analysis showed that high FOXC1 protein expression was an independent prognostic factor for NSCLC patients. Our study suggests that over-expression of FOXC1 may play an important role in the progression of NSCLC, and FOXC1 expression may offer a valuable marker for predicting the outcome of patients with NSCLC.
本研究旨在检测叉头框蛋白C1（FOXC1）在人非小细胞肺癌（NSCLC）中的表达，并分析其与NSCLC患者预后的相关性。采用定量实时聚合酶链反应（PCR）和蛋白质免疫印迹法检测30例NSCLC组织及相应癌旁组织样本中FOXC1 mRNA和蛋白的表达水平。应用免疫组织化学法检测125例NSCLC组织中FOXC1的表达。我们发现，NSCLC组织中FOXC1 mRNA和蛋白的表达水平显著高于相应癌旁组织。FOXC1高表达与肿瘤低分化、肿瘤-淋巴结-转移（TNM）分期及淋巴结转移相关。FOXC1高表达患者的总生存率低于低表达患者。多因素分析显示，FOXC1蛋白高表达是NSCLC患者的独立预后因素。我们的研究提示，FOXC1过表达可能在NSCLC进展中起重要作用，且FOXC1表达可能为预测NSCLC患者的预后提供有价值的标志物。
