Li Hong, Zhou Ren-Jie, Zhang Guo-Qiang, Xu Jian-Ping
Department of Anesthesiology, Xinqiao Hospital, Third Military Medical University, No. 183, Xinqiaozheng Rd, Chongqing, 400037, China.
Tumour Biol. 2013 Jun;34(3):1807-12. doi: 10.1007/s13277-013-0720-4. Epub 2013 Mar 8.
This study was designed to evaluate expression and prognostic significance of runt-related transcription factor (RUNX)-2 in human nonsmall cell lung cancer (NSCLC). RUNX2 expression was examined at mRNA and protein levels by real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blot in NSCLC tissues and adjacent normal tissues. In addition, RUNX2 expression was analyzed by immunohistochemistry in 121 clinicopathologically characterized NSCLC cases. The relationship between the expression of RUNX2 and clinicopathological characteristics and prognosis was statistically analyzed. Both qRT-PCR and Western blot demonstrated that RUNX2 mRNA and protein levels were significantly higher in NSCLC tissues compared to the adjacent normal tissues from the same individual. Immunohistochemistry analysis showed that RUNX2 expression was significantly correlated with tumor size, tumor stage, and lymph node metastasis. Higher RUNX2 expression was associated with shorter postoperative survival time of NSCLC patients by Kaplan-Meier method and was found to be an independent risk factor that influences the postoperative survival time of NSCLC patients by Cox regression analysis. In conclusion, these data showed that RUNX2 may play an important role in NSCLC tumorigenesis, and RUNX2 might serve as a novel prognostic marker in NSCLC.
本研究旨在评估矮小相关转录因子（RUNX）-2在人非小细胞肺癌（NSCLC）中的表达及其预后意义。采用实时定量逆转录聚合酶链反应（qRT-PCR）和蛋白质免疫印迹法，检测NSCLC组织及其癌旁正常组织中RUNX2在mRNA和蛋白质水平的表达情况。此外，运用免疫组织化学方法分析121例具有临床病理特征的NSCLC病例中RUNX2的表达。对RUNX2表达与临床病理特征及预后的关系进行统计学分析。qRT-PCR和蛋白质免疫印迹法均显示，与同一个体的癌旁正常组织相比，NSCLC组织中RUNX2的mRNA和蛋白质水平显著更高。免疫组织化学分析表明，RUNX2表达与肿瘤大小、肿瘤分期及淋巴结转移显著相关。通过Kaplan-Meier法分析发现，RUNX2高表达与NSCLC患者术后生存时间缩短相关，且经Cox回归分析表明，RUNX2是影响NSCLC患者术后生存时间的独立危险因素。总之，这些数据表明RUNX2可能在NSCLC的肿瘤发生过程中发挥重要作用，并且RUNX2可能作为NSCLC一种新的预后标志物。
