College of Animal Science and Technology, Agricultural University of Hebei, 289 Lingyusi Street, Baoding, 071001, China.
Biochem Genet. 2013 Apr;51(3-4):211-22. doi: 10.1007/s10528-012-9556-7. Epub 2012 Dec 21.
Polymorphism of the prion protein gene (PRNP) is usually associated with scrapie susceptibility or resistance. To determine the variability of PRNP in Chinese indigenous goat breeds, we isolated genomic DNA from goat blood and amplified and sequenced the coding region of the gene. We identified 10 polymorphic sites that gave rise to 28 haplotypes. Clear frequency differences were found between northern and southern breeds and confirmed by genetic distance analysis, except for the Tangshan dairy goat. Phylogeographic analysis supported the idea that northern and southern breeds might be considered separate clusters, except for the Tangshan dairy goat. The finding of significant differences in allele distribution in northern and southern goats, especially if involved in modulating resistance/susceptibility, needs to be carefully considered for the feasibility of selection plans for resistance to scrapie.
朊病毒蛋白基因（PRNP）的多态性通常与羊瘙痒病的易感性或抗性相关。为了确定中国本土山羊品种中 PRNP 的变异性，我们从山羊血液中分离基因组 DNA，并扩增和测序了该基因的编码区。我们鉴定出了 10 个导致 28 种单倍型的多态性位点。除了唐山奶山羊外，通过遗传距离分析发现，北方和南方品种之间存在明显的频率差异。系统发生地理分析支持了这样一种观点，即北方和南方品种可能被视为单独的聚类，除了唐山奶山羊。如果涉及调节抗性/易感性，需要仔细考虑北方和南方山羊等位基因分布存在显著差异的情况，以评估对羊瘙痒病抗性的选择计划的可行性。
