Department of Clinical Immunology, Copenhagen University Hospital Blegdamsvej 9, DK-2100 Copenhagen Ø, Denmark.
J Infect Dis. 2013 Mar 15;207(6):903-6. doi: 10.1093/infdis/jis924. Epub 2012 Dec 21.
We aimed to evaluate whether the HLA-G 14-base pair (bp) polymorphism (rs16375) has an impact on human immunodeficiency virus HIV progression and survival in an antiretroviral therapy-naive Zimbabwean cohort (n = 312). Rs16375 was genotyped using a competitive allele-specific polymerase chain reaction system; CD4 cell counts and HIV RNA were measured with flow cytometry and commercially available polymerase chain reaction; survival was followed up for 4.3 years. The homozygous HLA-G -14-bp genotype is associated with higher viral load (P = .004), lower CD4 cell count (P = .01), and increased mortality (hazard ratio, 1.9; 95% confidence interval, 1.033-3.522; P = .04) compared with HLA-G +14-bp carriers.
我们旨在评估 HLA-G 14 碱基对（bp）多态性（rs16375）是否对未经抗逆转录病毒治疗的津巴布韦队列（n=312）中的人类免疫缺陷病毒（HIV）进展和生存产生影响。使用竞争性等位基因特异性聚合酶链反应系统对 rs16375 进行基因分型；通过流式细胞术和商业聚合酶链反应测量 CD4 细胞计数和 HIV RNA；随访 4.3 年。与 HLA-G +14-bp 携带者相比，纯合 HLA-G -14-bp 基因型与更高的病毒载量（P=.004）、更低的 CD4 细胞计数（P=.01）和更高的死亡率（危险比，1.9；95%置信区间，1.033-3.522；P=.04）相关。
