Department of Clinical Immunology, Copenhagen University Hospital, Denmark.
Infect Genet Evol. 2012 Jul;12(5):1087-93. doi: 10.1016/j.meegid.2012.03.013. Epub 2012 Mar 29.
The C-C motif chemokine ligand 3-like (CCL3L) protein is a potent chemoattractant which by binding to C-C chemokine receptor type 5 (CCR5) inhibits human immunodeficiency virus (HIV) entry. Copy number variation (CNV) of the CCL3L has been shown to be associated with HIV susceptibility and progression to AIDS, but these results have been inconsistent. We examined a Zimbabwean study population for an association of CCL3L CNV with HIV status, progression (CD4 T-cells and viral load), and survival. Another aim was to investigate the possible effects of CCL3L CNV on CCL3 protein concentration. A treatment-naïve cohort, which included 153 HIV infected and 159 HIV uninfected individuals, was followed for up to 4.3 years. The CNV of the CCL3L was determined by duplex real-time polymerase chain reaction. We found no association between four CCL3L CNV strata and HIV status (P=0.7), CD4 T-cell count (P=0.9), viral load (P=0.9), or CCL3 protein levels (P=1.0). Survival among the HIV infected individuals did not differ according to CCL3L copy number. In this cohort, CCL3L CNV did not affect HIV status, pathogenesis, or survival.
CC 趋化因子配体 3 样蛋白（CCL3L）是一种有效的趋化因子，通过与 C 型趋化因子受体 5（CCR5）结合来抑制人类免疫缺陷病毒（HIV）进入细胞。CCL3L 的拷贝数变异（CNV）与 HIV 易感性和艾滋病进展有关，但这些结果并不一致。我们对津巴布韦的研究人群进行了研究，以确定 CCL3L CNV 与 HIV 状态、进展（CD4 T 细胞和病毒载量）和生存的关联。另一个目的是研究 CCL3L CNV 对 CCL3 蛋白浓度的可能影响。我们对一个未经治疗的队列进行了随访，该队列包括 153 名 HIV 感染者和 159 名 HIV 未感染者，随访时间长达 4.3 年。CCL3L 的 CNV 通过双路实时聚合酶链反应确定。我们发现，四个 CCL3L CNV 层与 HIV 状态（P=0.7）、CD4 T 细胞计数（P=0.9）、病毒载量（P=0.9）或 CCL3 蛋白水平（P=1.0）之间均无关联。HIV 感染者的生存率也与 CCL3L 拷贝数无关。在这个队列中，CCL3L CNV 并未影响 HIV 状态、发病机制或生存。
