Department of Laboratory Medicine, Gangneung Asan Hospital, University of Ulsan College of Medicine, Gangneung, South Korea.
J Clin Microbiol. 2013 Mar;51(3):1008-13. doi: 10.1128/JCM.02495-12. Epub 2012 Dec 26.
A multiplex real-time PCR assay that simultaneously detects the mecA, staphylococcal cassette chromosome (SCCmec)-open reading frame X (orfX) junction, and staphylococcal 16S rRNA genes was developed and evaluated using 444 staphylococcal strains. We demonstrated that this assay resulted in fewer false-positive results than a single-locus real-time PCR assay that amplified the SCCmec-orfX junction. This assay would be useful in a clinical laboratory in a region of high endemicity for methicillin-resistant Staphylococcus aureus (MRSA) infections.
我们开发并评估了一种多重实时 PCR 检测方法,该方法可同时检测 mecA、葡萄球菌盒式染色体(SCCmec)-开放阅读框 X(orfX)连接点和葡萄球菌 16S rRNA 基因,使用了 444 株葡萄球菌菌株。我们证明,与扩增 SCCmec-orfX 连接点的单基因实时 PCR 检测方法相比,该检测方法导致的假阳性结果更少。在耐甲氧西林金黄色葡萄球菌(MRSA)感染高发地区的临床实验室中,这种检测方法将非常有用。