University of Ottawa, Ottawa, ON, Canada.
The Ottawa Hospital Research Institute (OHRI), Ottawa, ON, Canada.
Sci Rep. 2022 Jan 25;12(1):1337. doi: 10.1038/s41598-022-05246-3.
Delayed entry of blood culture bottles is frequent in consolidated laboratories. A retrospective study evaluated time from insertion to detection and total detection time as a function of preincubation time, and we prospectively looked for false negative results. 69,604 blood culture bottles were reviewed for preincubation time, incubation time and total detection time. Positive cultures for specific bacterial subtypes were reviewed to assess the effect of preincubation time on likelihood of detection. 492 negative blood cultures were prospectively tested by 16S RNA PCR and Staphylococcus-specific PCR for the presence of bacterial DNA. Mean preincubation time for samples collected within the city-limits was 3.94 h versus 9.49-18.89 h for other client sites. Higher preincubation times were partially mitigated by a lower incubation time, with an overall increase in total detection time. A lower odds ratio of recovery of Staphylococcus spp was identified, but not confirmed by terminal subcultures and molecular assays. Prolonged preincubation of blood cultures affects total detection time despite a reduction in incubation time. Successful centralization of microbiological services may depend upon optimization of courier routes for inoculated blood culture bottles. Our data supports consideration for an increase in suggested maximum preincubation times.
在整合的实验室中，血液培养瓶的延迟进入很常见。一项回顾性研究评估了从插入到检测和总检测时间作为预孵育时间的函数，我们前瞻性地寻找假阴性结果。对 69604 个血培养瓶的预孵育时间、孵育时间和总检测时间进行了回顾。对特定细菌亚型的阳性培养物进行了回顾，以评估预孵育时间对检测可能性的影响。492 份阴性血培养物通过 16S RNA PCR 和葡萄球菌特异性 PCR 前瞻性检测细菌 DNA 的存在。在城市范围内采集的样本的平均预孵育时间为 3.94 小时，而其他客户站点的预孵育时间为 9.49-18.89 小时。较低的孵育时间部分缓解了较高的预孵育时间，总检测时间总体增加。虽然终端亚培养和分子检测未能证实，但鉴定出金黄色葡萄球菌回收的可能性降低。尽管孵育时间缩短，但血液培养物的长时间预孵育会影响总检测时间。成功集中化微生物服务可能取决于优化接种血培养瓶的快递路线。我们的数据支持考虑增加建议的最大预孵育时间。
