堪萨斯州和密苏里州首次报告采采蝇（双翅目：Psychodidae），以及一种区分 Lutzomyia shannoni 和 Lutzomyia vexator 的 PCR 方法。
First report of phlebotomine sand flies (Diptera: Psychodidae) in Kansas and Missouri, and a PCR method to distinguish Lutzomyia shannoni from Lutzomyia vexator.
机构信息
Department of Entomology, Kansas State University, Manhattan, 66506, USA.
出版信息
J Med Entomol. 2012 Nov;49(6):1460-5. doi: 10.1603/me12105.
Sand flies Lutzomyia (Psathyromyia) shannoni (Dyar) and Lu. (Helcocyrtomyia) vexator (Coquillet) were collected for the first time in southwest Missouri and southeast Kansas, expanding the known range of these species in North America. Altogether, 680 sand flies (356 males and 324 females) were collected during trapping from May through October 2011 and identified using morphological characters. Of the total sand flies collected, 315 were identified as Lu. shannoni, with 181 individuals (or 26.6% of all sand flies) trapped in Missouri and 134 individuals (or 19.7%) trapped in Kansas. Whereas 358 Lu. vexator were identified from southwest Missouri, only a single specimen was trapped in southeast Kansas. One male Lu. vexator with asymmetric gonostyli was trapped in Missouri. We also developed a polymerase chain reaction protocol to consistently and accurately distinguish Lu. shannoni from Lu. vexator based on presence or absence of a 416 bp fragment from the cytochrome oxidase c subunit 1 gene.
首次在密苏里州西南部和堪萨斯州东南部采集到沙蝇 Lutzomyia (Psathyromyia) shannoni (Dyar) 和 Lu. (Helcocyrtomyia) vexator (Coquillet)，这使得这些物种在北美的分布范围进一步扩大。2011 年 5 月至 10 月期间，通过诱捕共采集了 680 只沙蝇（356 只雄性和 324 只雌性），并通过形态特征进行了鉴定。在所采集的沙蝇中，有 315 只被鉴定为 Lu. shannoni，其中 181 只（占所有沙蝇的 26.6%）在密苏里州捕获，134 只（占 19.7%）在堪萨斯州捕获。从密苏里州西南部采集到 358 只 Lu. vexator，而在堪萨斯州东南部仅捕获到一只。在密苏里州捕获到一只雄性 Lu. vexator，其性二型特征明显。我们还开发了一种聚合酶链反应方案，根据细胞色素氧化酶 c 亚基 1 基因的存在或缺失，能够始终如一地准确区分 Lu. shannoni 和 Lu. vexator。
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