Uniformed Services University of the Health Sciences, Department of Preventive Medicine and Biometrics, 4301 Jones Bridge Road, Bethesda, MD 20814-4799, USA.
J Med Entomol. 2011 Mar;48(2):154-66. doi: 10.1603/me10199.
A morphometric and molecular study of adult male and female Lutzomyia shannoni (Dyar 1929) collected at seven different locations within the southeastern United States was conducted to assess the degree of divergence between the grouped specimens from each location. The collection locations were as follows: Fort Bragg, NC; Fort Campbell, KY; Fort Rucker, AL; Ossabaw Island, GA; Patuxent National Wildlife Research Refuge, MD; Suwannee National Wildlife Refuge, FL; and Baton Rouge, LA. Forty males and forty females from each location were analyzed morphometrically from 54 and 49 character measurements, respectively. In addition, the molecular markers consisting of the partial cytochrome c oxidase subunit I (from 105 sand flies: 15 specimens/collection site) and the partial internal transcribed spacer 2 (from 42 sand flies: six specimens/collection site) were compared. Multivariate analyses indicate that the low degree of variation between the grouped specimens from each collection site prevents the separation of any collection site into an entity that could be interpreted as a distinct population. The molecular analyses were in concordance with the morphometric study as no collection location grouped into a separate population based on the two partial markers. The grouped specimens from each collection site appear to be within the normal variance of the species, indicating a single population in the southeast United States. It is recommended that additional character analyses of L. shannoni based on more molecular markers, behavioral, ecological, and physiological characteristics, be conducted before ruling out the possibility of populations or a cryptic species complex within the southeastern United States.
对在美国东南部七个不同地点采集的成年雄性和雌性 Lutzomyia shannoni（Dyar 1929）进行了形态和分子研究，以评估来自每个地点的分组标本之间的分歧程度。采集地点如下：北卡罗来纳州布拉格堡；肯塔基州坎贝尔堡；阿拉巴马州鲁克瑟姆堡；乔治亚州奥萨巴岛；马里兰州帕塔克森特国家野生动物研究保护区；佛罗里达州斯旺尼国家野生动物保护区；以及路易斯安那州巴吞鲁日。从每个地点分别分析了 40 只雄性和 40 只雌性的 54 和 49 个特征测量值。此外，还比较了由部分细胞色素 c 氧化酶亚基 I（来自 105 只沙蝇：每个采集点 15 只标本）和部分内部转录间隔区 2（来自 42 只沙蝇：每个采集点 6 只标本）组成的分子标记。多变量分析表明，来自每个采集点的分组标本之间的差异程度很低，无法将任何采集点分离成可以解释为独特种群的实体。分子分析与形态学研究一致，因为没有任何采集地点根据两个部分标记分为单独的种群。来自每个采集点的分组标本似乎在该物种的正常变异范围内，表明在美国东南部存在一个单一的种群。建议在排除美国东南部存在种群或隐种复合体的可能性之前，基于更多分子标记、行为、生态和生理特征对 L. shannoni 进行额外的特征分析。
