通过脉冲和连续波超声对伊乐藻叶片中的细胞进行裂解。

Lysis of cells in Elodea leaves by pulsed and continuous wave ultrasound.

作者信息

Carstensen E L, Child S Z, Crane C, Miller M W, Parker K J

机构信息

Department of Electrical Engineering, University of Rochester, NY 14627.

出版信息

Ultrasound Med Biol. 1990;16(2):167-73. doi: 10.1016/0301-5629(90)90145-3.

DOI:10.1016/0301-5629(90)90145-3

PMID:2327043

Abstract

Resonance lysis of the cells in the leaves of the aquatic plant Elodea originally reported by Miller have thresholds at intensities of a few W/cm2. With pulsed ultrasound, the resonance behavior vanishes and the thresholds are at much higher amplitudes. This is similar to the characteristics of the thresholds for killing of Drosophila larvae by pulsed ultrasound. Both organisms contain small gas bodies within the tissues which may serve as nuclei for a cavitation related phenomenon. The results suggest that the response of these bubbles to continuous wave fields and very short pulses is qualitatively different.

摘要

最初由米勒报道的水生植物伊乐藻叶片中细胞的共振裂解，其阈值在几瓦每平方厘米的强度。对于脉冲超声，共振行为消失，阈值处于高得多的振幅。这类似于脉冲超声杀死果蝇幼虫的阈值特征。两种生物体在组织内都含有小的气体泡，这些气体泡可能作为与空化相关现象的核心。结果表明，这些气泡对连续波场和极短脉冲的响应在性质上是不同的。