H. E. J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi-75270, Pakistan.
Life Sci. 2013 Feb 27;92(3):187-92. doi: 10.1016/j.lfs.2012.11.022. Epub 2012 Dec 24.
One of the aims of this research work is the isolation and identification of various constituents of two medicinally important plants (Iris loczyi and Iris unguicularis). Secondly, the prime aim is the biological evaluation of these natural products to discover new potential inhibitors of α-glucosidase enzyme and protein glycation.
Plants of the genus Iris contain a variety of secondary metabolites. Chromatographic techniques were applied for the isolation of different compounds from Iris loczyi and Iris unguicularis. All the isolated compounds were screened for their α-glucosidase enzyme inhibition and antiglycation potential.
It is shown in the results that two compounds (Kaempferol and 8-Methoxyeriodictyol) isolated from plant Iris unguicularis and compounds (Arborinone and 5,7-dihydroxy-2',6-dimethoxyisoflavone) isolated from plant Iris loczyi possess promising activity against α-glucosidase enzyme as compare to acarbose which is used as a standard α-glucosidase inhibitor in this study. A flavanone (2',5-dihydroxy-6,7-methylenedioxy) isolated from Iris loczyi was explored as most active anti-glycating agent.
α-Glucosidase enzyme is a therapeutic target to treat carbohydrate mediated diseases. In this study various inhibitors of α-glucosidase are identified which might be important for the management of diabetes. Similarly, antiglycation agents may have application for the management of late diabetic complications.
本研究工作的目的之一是分离和鉴定两种药用植物（鸢尾和鸢尾）的各种成分。其次，主要目的是对这些天然产物进行生物评估，以发现新的α-葡萄糖苷酶抑制剂和蛋白质糖化抑制剂。
鸢尾属植物含有多种次生代谢产物。应用色谱技术从鸢尾和鸢尾中分离出不同的化合物。所有分离出的化合物均进行α-葡萄糖苷酶抑制作用和抗糖化潜力的筛选。
结果表明，从植物鸢尾中分离出的两种化合物（山奈酚和 8-甲氧基杨梅素）以及从植物鸢尾中分离出的两种化合物（阿伯酮和 5,7-二羟基-2',6-二甲氧基异黄酮）对α-葡萄糖苷酶具有有前途的活性，与本研究中用作标准α-葡萄糖苷酶抑制剂的阿卡波糖相比。从鸢尾中分离出的一种黄烷酮（2',5-二羟基-6,7-亚甲二氧基）被发现是最有效的糖化抑制剂。
α-葡萄糖苷酶是治疗碳水化合物介导疾病的治疗靶点。在这项研究中，鉴定出了各种α-葡萄糖苷酶抑制剂，这对于糖尿病的治疗可能很重要。类似地，抗糖化剂可能可用于治疗晚期糖尿病并发症。
