Chemical proteomics: ligation and cleavage of protein modifications.

Bioorthogonal ligation and cleavage methods are of major importance in the field of chemical biology. Recently, there has been significant progress in the improvement of classic ligation procedures as well as in the establishment of new ligation methodologies. Furthermore, the design of cleavable linkers for protein enrichment has lately received much attention. These techniques equip researchers with a wealth of tools suitable for proteomic applications. In order to ease navigation through these diverse systems, we here provide a comprehensive overview of methods that are useful for chemical proteomics.