Chair of Organic Chemistry II, Department of Chemistry, Technical University of Munich, Garching 85747, Germany.
Curr Opin Chem Biol. 2013 Feb;17(1):110-7. doi: 10.1016/j.cbpa.2012.11.007. Epub 2012 Dec 28.
Bioorthogonal ligation and cleavage methods are of major importance in the field of chemical biology. Recently, there has been significant progress in the improvement of classic ligation procedures as well as in the establishment of new ligation methodologies. Furthermore, the design of cleavable linkers for protein enrichment has lately received much attention. These techniques equip researchers with a wealth of tools suitable for proteomic applications. In order to ease navigation through these diverse systems, we here provide a comprehensive overview of methods that are useful for chemical proteomics.
生物正交连接和切割方法在化学生物学领域具有重要意义。最近，经典连接程序的改进以及新连接方法的建立都取得了重大进展。此外，用于蛋白质富集的可切割接头的设计最近也受到了广泛关注。这些技术为研究人员提供了丰富的适用于蛋白质组学应用的工具。为了方便在这些不同的系统中进行导航，我们在这里提供了一个对化学蛋白质组学有用的方法的全面概述。
