Wang Lan, Xia Menglei, Zhang Lianhua, Chen Hongzhang
State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, China.
World J Microbiol Biotechnol. 2014 Jul;30(7):1969-76. doi: 10.1007/s11274-014-1619-y. Epub 2014 Feb 9.
An unexpected promotion effect of Ginkgo leaf on the growth of Clostridium acetobutylicum ATCC 824 and acetone-butanol-ethanol (ABE) fermentation was investigated. Component analysis of Ginkgo leaf was carried out and flavonoids were determined as the potential key metabolites. Then the flavonoids feeding experiments were carried out. Results showed that addition of only 10 mg/L flavonoids to the fermentation broth can promote butanol and ABE titre up to 14.5 and 17.8 g/L after 5 days of fermentation, that is, 74 and 68% higher than the control. A 2.2-fold biomass also has been achieved. Furthermore, by employing such novel founding, we easily exploited flavonoids from soybean and some agriculture wastes as the wide-distributed and economic feasible ABE fermentation promoter. The mechanism of the above effects was investigated from the perspective of oxidation-reduction potential. This work opens a new way in the efforts to increase the titer of butanol.
研究了银杏叶对丙酮丁醇梭菌ATCC 824生长及丙酮-丁醇-乙醇（ABE）发酵的意外促进作用。对银杏叶进行了成分分析，并确定黄酮类化合物为潜在的关键代谢产物。然后进行了黄酮类化合物添加实验。结果表明，在发酵液中仅添加10 mg/L黄酮类化合物，发酵5天后丁醇和ABE产量分别可达14.5 g/L和17.8 g/L，分别比对照高74%和68%。生物量也提高了2.2倍。此外，基于这一新发现，我们成功开发了从大豆和一些农业废弃物中提取黄酮类化合物作为广泛分布且经济可行的ABE发酵促进剂的方法。从氧化还原电位的角度研究了上述作用的机制。这项工作为提高丁醇产量开辟了一条新途径。
