小乙型肝炎表面抗原与肝癌细胞中的烯酰辅酶 A 水合酶相互作用并调节其表达。
Small hepatitis B surface antigen interacts with and modulates enoyl-coenzyme A hydratase expression in hepatoma cells.
机构信息
Center of Liver Diseases, The First Affiliated Hospital of Fujian Medical University, Fuzhou 350004, Fujian, China.
出版信息
Arch Virol. 2013 May;158(5):1065-70. doi: 10.1007/s00705-012-1581-7. Epub 2012 Dec 30.
Enoyl-coenzyme A hydratase (ECHS1) is a key enzyme in the metabolism of fatty acids in mitochondria. We previously reported that hepatitis B surface antigen (HBsAg) interacted with ECHS1 in a yeast two-hybrid system. In the current study, we further examined their interaction by using GST pull-down and co-immunoprecipitation assays. The results confirmed that small hepatitis B surface antigen (SHBs) interacted with ECHS1. Furthermore, confocal imaging showed that SHBs and ECHS1 co-localized in HepG2 cells. To clarify the biological function of the interaction, human hepatoma cell lines that transiently and stably expressed SHBs were generated. The expression of SHBs led to a significant decrease in ECHS1 protein levels. ECHS1 protein levels were reduced to 48.44 ± 7.12 % in Huh7 cells transiently expressing SHBs, and to 54.97 ± 3.54 % in HepG2 cells stably expressing SHBs. In conclusion, our findings suggest that SHBs interacts with ECHS1 and regulates ECHS1 protein levels in hepatoma cells.
烯酰辅酶 A 水合酶（ECHS1）是线粒体脂肪酸代谢中的关键酶。我们之前的研究报告表明，乙型肝炎表面抗原（HBsAg）在酵母双杂交系统中与 ECHS1 相互作用。在本研究中，我们进一步通过 GST 下拉和共免疫沉淀实验来检测它们的相互作用。结果证实小乙型肝炎表面抗原（SHBs）与 ECHS1 相互作用。此外，共聚焦成像显示 SHBs 和 ECHS1 在 HepG2 细胞中共定位。为了阐明相互作用的生物学功能，我们生成了瞬时和稳定表达 SHBs 的人肝癌细胞系。SHBs 的表达导致 ECHS1 蛋白水平显著下降。在瞬时表达 SHBs 的 Huh7 细胞中，ECHS1 蛋白水平下降至 48.44±7.12%，在稳定表达 SHBs 的 HepG2 细胞中，ECHS1 蛋白水平下降至 54.97±3.54%。总之，我们的研究结果表明，SHBs 与 ECHS1 相互作用，并调节肝癌细胞中的 ECHS1 蛋白水平。
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