Department of Gastroenterology, Zhongshan Hospital Affiliated to Xiamen University, 201 Hubin South Road, Xiamen 361004, Fujian Province, China.
Cancer Lett. 2013 Mar 1;330(1):67-73. doi: 10.1016/j.canlet.2012.11.030. Epub 2012 Nov 23.
We aimed to confirm the role of ECHS1 as a binding protein of HBsAg (HBs) and investigate its function during the development of hepatocellular carcinoma (HCC). Our results show that both exogenous and endogenous ECHS1 proteins bind to HBs and co-localize in the cytoplasm in vitro. The coexistence of HBs and ECHS1 enhances HepG2 cell apoptosis, affects ECHS1 localization in the mitochondria and induces apoptosis by decreasing the mitochondrial membrane potential (MMP). These findings suggest that ECHS1 may be applied as a potential therapeutic target during the treatment of HBV-related hepatitis or HCC.
我们旨在确认 ECHS1 作为 HBsAg（HBs）结合蛋白的作用，并研究其在肝细胞癌（HCC）发展过程中的功能。我们的结果表明，外源性和内源性 ECHS1 蛋白都与 HBs 结合，并在体外共定位于细胞质中。HBs 和 ECHS1 的共存增强了 HepG2 细胞的凋亡，影响了 ECHS1 在线粒体中的定位，并通过降低线粒体膜电位（MMP）诱导凋亡。这些发现表明，ECHS1 可作为治疗 HBV 相关肝炎或 HCC 的潜在治疗靶点。
