Georgiou C A, Koupparis M A
Department of Chemistry, University of Athens, Greece.
Analyst. 1990 Mar;115(3):309-13. doi: 10.1039/an9901500309.
The reaction of para- and meta-substituted phenols with 1-nitroso-2-naphthol in the presence of either CeIV or PbIV as an oxidant has been used to develop a fast automated flow injection (Fl) method. A stopped-flow kinetic study of the reaction revealed the optimum conditions for the proposed Fl method. Acetaminophen, amoxicillin, cefadroxil, isoxsuprine, nylidrin, propylparaben, tyrosine and metaraminol can be determined in the range 1 x 10(-4)-8 x 10(-4) M, with relative standard deviations of less than 2%, and a measurement throughput of 40 measurements h-1. The method was evaluated by performing interference studies of common excipients and assaying commercial formulations of acetaminophen and isoxsuprine. The results were in good agreement with those obtained by acceptable spectrophotometric or high-performance liquid chromatographic methods (mean difference 2.1%). The high sample throughput of the Fl method was exploited by performing a content uniformity test of isoxsuprine tablets.
在铈（IV）或铅（IV）作为氧化剂存在的情况下，对-和间-取代酚与1-亚硝基-2-萘酚的反应已被用于开发一种快速自动流动注射（FI）方法。对该反应的停流动力学研究揭示了所提出的FI方法的最佳条件。对乙酰氨基酚、阿莫西林、头孢羟氨苄、异克舒令、尼立替林、对羟基苯甲酸丙酯、酪氨酸和间羟胺的测定范围为1×10⁻⁴ - 8×10⁻⁴ M，相对标准偏差小于2%，测量通量为40次测量/小时。通过对常见辅料进行干扰研究以及对乙酰氨基酚和异克舒令的商业制剂进行分析来评估该方法。结果与通过可接受的分光光度法或高效液相色谱法获得的结果高度一致（平均差异2.1%）。通过对异克舒令片剂进行含量均匀度测试，利用了FI方法的高样品通量。
