South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.
Chemosphere. 2013 Mar;91(1):28-34. doi: 10.1016/j.chemosphere.2012.11.038. Epub 2012 Dec 28.
Cadmium is a potentially toxic and carcinogenic nonessential heavy metal. This study investigated Cd accumulation along the soil-plant (Amaranthus hypochondriacus L.)-insect (Prodenia litura) food chain and the detoxification strategies at different trophic levels. A. hypochondriacus leaves could accumulate high levels of Cd from polluted soil. The Cd concentration in P. litura larvae increased with increasing Cd concentrations in the food plant. Transfer coefficients of Cd were high from soil to leaf and from larvae to feces. The leaves of A. hypochondriacus had the highest value of Cd accumulation in pectates and protein-integrated forms (extracted by 1M NaCl). Among all the subcellular fractions in larvae of P. litura, the heat-stable protein fraction was the dominant metal-binding compartment for Cd. The Cd subcellular level played an important role in Cd sequestration and excretion by P. liura larva feeding on Cd contaminated amaranth leaves. This is the first attempt to account for subcellular distribution associated with Cd in P. litura when interpreting Cd detoxification and transfer along insect food chain.
镉是一种潜在毒性和致癌的非必需重金属。本研究调查了土壤-植物（苋菜）-昆虫（斜纹夜蛾）食物链中镉的积累情况，以及不同营养水平的解毒策略。苋菜叶片可以从污染的土壤中积累高水平的镉。斜纹夜蛾幼虫体内的镉浓度随着食物植物中镉浓度的增加而增加。镉从土壤到叶片和从幼虫到粪便的转移系数较高。苋菜叶片中果胶和蛋白质整合形式（用 1M NaCl 提取）的镉积累值最高。在斜纹夜蛾幼虫的所有亚细胞 fractions 中，热稳定蛋白 fraction 是 Cd 的主要金属结合区室。斜纹夜蛾幼虫食用受镉污染的苋菜叶片时，Cd 的亚细胞水平在 Cd 的螯合和排泄中起着重要作用。这是首次尝试解释斜纹夜蛾幼虫中与 Cd 相关的亚细胞分布，以解释昆虫食物链中 Cd 的解毒和转移。
