School of Agriculture and Food Sciences, The University of Queensland, Gatton Campus, Gatton, 4343, Queensland, Australia.
Anim Reprod Sci. 2013 Feb;137(1-2):74-81. doi: 10.1016/j.anireprosci.2012.11.005. Epub 2012 Nov 16.
The aim was to elucidate the role of LH and FSH in testicular development and growth in the neonatal boar. On Day 10 after birth (Day 0 of study), animals were assigned to one of nine groups (n=6): Group 1, control, no treatment; Group 2, hemicastrated (H); Group 3, H and implanted with GnRH agonist (H+GnRH); Group 4, H+GnRH+FSH 200μg/kg daily from Days 0 to 14 (D0-14); Group 5, H+GnRH+FSH 400μg/kg D0-14; Group 6, H+GnRH+FSH 400μg/kg in PVP D0-14; Group 7, H+GnRH+LH 200μg/kg D0-14; Group 8, H+GnRH+LH 400μg/kg D0-14; Group 9, H+GnRH+LH and FSH 200μg/kg D0-14. The right testis in control and hemicastrated boars was removed on Day 15. Hemicastrated boars had greater (P<0.05) testicular growth than control boars and testicular growth was prevented in boars treated with GnRH agonist. FSH induced Sertoli cell proliferation but not testicular growth whilst LH induced Leydig cell proliferation and testicular growth was similar to control boars but less than hemicastrated boars. LH+FSH induced similar testicular growth as LH alone and neither LH and/or FSH supported testicular hypertrophy in hemicastrated boars. The findings show conclusively for the first time that LH and FSH respectively induce Leydig cell and Sertoli cell proliferation in the neonatal boar. LH additionally supports a normal increase in testicular size in the neonatal boar.
目的在于阐明 LH 和 FSH 在新生公猪睾丸发育和生长中的作用。在出生后第 10 天（研究的第 0 天），将动物分配到九个组之一（n=6）：第 1 组，对照组，不治疗；第 2 组，半去势（H）；第 3 组，H 并植入 GnRH 激动剂（H+GnRH）；第 4 组，H+GnRH+FSH 200μg/kg 每日从第 0 天到第 14 天（D0-14）；第 5 组，H+GnRH+FSH 400μg/kg D0-14；第 6 组，H+GnRH+FSH 400μg/kg 在 PVP D0-14；第 7 组，H+GnRH+LH 200μg/kg D0-14；第 8 组，H+GnRH+LH 400μg/kg D0-14；第 9 组，H+GnRH+LH 和 FSH 200μg/kg D0-14。在第 15 天，对照组和半去势公猪的右侧睾丸被切除。与对照组相比，半去势公猪的睾丸生长更大（P<0.05），而 GnRH 激动剂治疗阻止了睾丸生长。FSH 诱导支持细胞增殖，但不诱导睾丸生长，而 LH 诱导间质细胞增殖，睾丸生长与对照组相似，但小于半去势组。LH+FSH 诱导的睾丸生长与 LH 单独诱导的相似，并且 LH 和/或 FSH 均不能支持半去势公猪的睾丸肥大。这些发现首次明确表明，LH 和 FSH 分别诱导新生公猪的间质细胞和支持细胞增殖。LH 另外支持新生公猪睾丸大小的正常增加。
