Hur Jin, Stein Barry D, Lee John Hwa
Veterinary Public Health, College of Veterinary Medicine, Chonbuk National University, Jeonju 561-756, Republic of Korea.
Can J Vet Res. 2012 Jul;76(3):186-94.
In order to construct a novel vaccine candidate for preventing post-weaning diarrhea in swine, the individual genes for Escherichia coli K88ab, K88ac, FedA, and FedF fimbriae were inserted into a secretion plasmid pBP244 containing asd, lepB, secA, and secB. These were transformed into Salmonella Typhimurium Δlon ΔcpxR Δasd. Secretion of the individual recombinant fimbrial antigens was confirmed by immunoblot analysis. Groups 1 and 2 mice received a single oral dose of the vaccine mixture and S. Typhimurium carrying pBP244 only as a control, respectively. In groups 3 and 4, mice were primed and boosted with the vaccine mixture and S. Typhimurium carrying pBP244 only as a control, respectively. In general, all immunized mice had significantly increased serum immunoglobulin (Ig)G (P < 0.05) and intestinal secretory IgA against the individual fimbrial antigens compared with those mice in the control group. In the IgG2a and IgG1 titer assay, only IgG2a titer was increased in group 1, while both IgG2a and IgG1 titers were increased in group 3. Furthermore, the vaccine strains were not detected in the excreted feces of any immunized mice. Thus, the vaccine candidate can be highly immunogenic and be safe to the environment.
为构建一种预防猪断奶后腹泻的新型候选疫苗，将大肠杆菌K88ab、K88ac、FedA和FedF菌毛的单个基因插入到含有asd、lepB、secA和secB的分泌质粒pBP244中。将这些质粒转化到鼠伤寒沙门氏菌ΔlonΔcpxRΔasd中。通过免疫印迹分析确认了各个重组菌毛抗原的分泌情况。第1组和第2组小鼠分别口服单剂量的疫苗混合物和仅携带pBP244的鼠伤寒沙门氏菌作为对照。在第3组和第4组中，小鼠分别用疫苗混合物和仅携带pBP244的鼠伤寒沙门氏菌进行初次免疫和加强免疫作为对照。总体而言，与对照组小鼠相比，所有免疫小鼠针对各个菌毛抗原的血清免疫球蛋白（Ig）G显著增加（P<0.05），肠道分泌型IgA也增加。在IgG2a和IgG1滴度测定中，第1组仅IgG2a滴度增加，而第3组IgG2a和IgG1滴度均增加。此外，在任何免疫小鼠的粪便中均未检测到疫苗菌株。因此，该候选疫苗具有高度免疫原性且对环境安全。
