College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China.
Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China.
Biomolecules. 2023 Nov 30;13(12):1726. doi: 10.3390/biom13121726.
Edema disease (ED) is a severe and lethal infectious ailment in swine, stemming from Shiga-toxin-producing (STEC). An efficient, user-friendly, and safe vaccine against ED is urgently required to improve animal welfare and decrease antibiotic consumption. Recombinant attenuated vaccines (RASV) administered orally induce both humoral and mucosal immune responses to the immunizing antigen. Their potential for inducing protective immunity against ED is significant through the delivery of STEC antigens. rSC0016 represents an enhanced recombinant attenuated vaccine vector designed for enterica serotype Choleraesuis. It combines sopB mutations with a regulated delay system to strike a well-balanced equilibrium between host safety and immunogenicity. We generated recombinant vaccine strains, namely rSC0016 (pS-FedF) and rSC0016 (pS-rStx2eA), and assessed their safety and immunogenicity in vivo. The findings demonstrated that the mouse models immunized with rSC0016 (pS-FedF) and rSC0016 (pS-rStx2eA) generated substantial IgG antibody responses to FedF and rStx2eA, while also provoking robust mucosal and cellular immune responses against both FedF and rStx2eA. The protective impact of rSC0016 (pS-FedF) against Shiga-toxin-producing surpassed that of rSC0016 (pS-rStx2eA), with percentages of 83.3%. These findings underscore that FedF has greater suitability for vaccine delivery via recombinant attenuated vaccines (RASVs). Overall, this study provides a promising candidate vaccine for infection with STEC.
水肿病（ED）是一种严重且致命的猪传染性疾病，源于产志贺毒素大肠杆菌（STEC）。为了提高动物福利和减少抗生素的使用，急需开发一种高效、易用且安全的 ED 疫苗。口服施用的重组减毒疫苗（RASV）可诱导针对免疫原的体液和黏膜免疫应答。通过递送 STEC 抗原，它们在诱导针对 ED 的保护性免疫方面具有重要潜力。rSC0016 是一种针对肠出血性大肠杆菌血清型志贺毒素的增强型重组减毒疫苗载体，它结合了 sopB 突变和调控延迟系统，在宿主安全性和免疫原性之间取得了良好的平衡。我们生成了重组疫苗株，即 rSC0016（pS-FedF）和 rSC0016（pS-rStx2eA），并在体内评估了它们的安全性和免疫原性。结果表明，用 rSC0016（pS-FedF）和 rSC0016（pS-rStx2eA）免疫的小鼠模型对 FedF 和 rStx2eA 产生了大量 IgG 抗体应答，同时还引发了针对 FedF 和 rStx2eA 的强大黏膜和细胞免疫应答。rSC0016（pS-FedF）对产志贺毒素大肠杆菌的保护作用优于 rSC0016（pS-rStx2eA），达到 83.3%。这些发现表明，FedF 更适合通过重组减毒疫苗（RASV）进行疫苗传递。总体而言，这项研究为 STEC 感染提供了一种有前途的候选疫苗。
