Beckett G J, Nicol F, Proudfoot D, Dyson K, Loucaides G, Arthur J R
University Department of Clinical Chemistry, Royal Infirmary, Edinburgh, U.K.
Biochem J. 1990 Mar 15;266(3):743-7. doi: 10.1042/bj2660743.
Selenium (Se) deficiency for 5 weeks in rats produced changes in the activity of a number of hepatic, renal and plasma enzymes. In animals whose food intake was restricted to 75% of normal for 2 weeks, Se deficiency produced significant increases in the activity of hepatic cytosolic 'malic' enzyme and mitochondrial alpha-glycerophosphate dehydrogenase (GPD), two enzymes that are particular sensitive to the thyroid-hormone concentrations in tissue. Propylthiouracil-induced hypothyroidism produced significant decreases in 'malic' enzyme and GPD activities. The effect of hypothyroidism on the activity of 'malic' enzyme, GPD and other enzymes studied in liver and plasma was often opposite to that seen in Se deficiency. Glutathione S-transferase (GST) activity was increased by both Se deficiency and hypothyroidism, but in hypothyroid animals further significant increases in GST were produced by Se deficiency. These data suggest that the changes in enzyme expression observed in Se deficiency are not caused by decreased tissue exposure to thyroid hormones.
大鼠缺硒5周会导致多种肝脏、肾脏和血浆酶的活性发生变化。在食物摄入量被限制为正常水平75%达2周的动物中，缺硒会使肝脏胞质“苹果酸”酶和线粒体α-甘油磷酸脱氢酶（GPD）的活性显著增加，这两种酶对组织中的甲状腺激素浓度特别敏感。丙基硫氧嘧啶诱导的甲状腺功能减退会使“苹果酸”酶和GPD活性显著降低。甲状腺功能减退对肝脏和血浆中“苹果酸”酶、GPD及其他所研究酶活性的影响往往与缺硒时相反。谷胱甘肽S-转移酶（GST）的活性在缺硒和甲状腺功能减退时均会增加，但在甲状腺功能减退的动物中，缺硒会使GST进一步显著增加。这些数据表明，在缺硒时观察到的酶表达变化并非由组织对甲状腺激素的暴露减少所致。
