Reiter R, Wendel A
Biochem Pharmacol. 1985 Jul 1;34(13):2287-90. doi: 10.1016/0006-2952(85)90783-x.
Male mice were fed a torula yeast-based diet containing different amounts of added selenium for a period of 4 months. Liver glutathione peroxidase activity assayed with H2O2 showed a logarithmic dependence on dietary selenium with a saturation plateau above 2 ppm Se and an extrapolated zero of 0.02 ppm Se. In contrast, liver selenium content and GSH-Peroxidase activity showed a linear correlation. Glutathione peroxidase activity became undetectable at a liver Se content of about 90 ng Se/g liver wet wt. Thus, about 10% of liver selenium is not related to GSH-Px activity. Five dietary groups were supplemented, respectively, with 0, 0.05, 0.5, 5.0 and 10 ppm Se in the form of Na2SeO3. Some changes in drug metabolism enzymes were observed with the high Se diets. An increase occurred in Non-Se-GSH activity as well as in ethacrynic acid-assayed GSH transferase, these are interpreted as early signs of Se toxicity. The diet containing 0.01 ppm Se with no supplementary Se produced the multiple hepatic enzyme modulations which were previously reported. The animals raised on this very low Se diet had normal hepatic contents of glutathione, alpha-tocopherol, calcium, magnesium, iron, zinc, copper and manganese compared to controls supplemented with 0.5 ppm Se. However, significant changes in the microsomal fatty acid pattern were observed while the total phospholipid content as well as membrane fluidity showed no differences between the two dietary groups.
雄性小鼠被喂食以圆酵母为基础、添加了不同量硒的饲料，持续4个月。用H2O2测定的肝脏谷胱甘肽过氧化物酶活性显示，其对膳食硒呈对数依赖性，在硒含量高于2 ppm时出现饱和平台，外推零值为0.02 ppm硒。相比之下，肝脏硒含量与谷胱甘肽过氧化物酶活性呈线性相关。当肝脏硒含量约为90 ng硒/克肝脏湿重时，谷胱甘肽过氧化物酶活性无法检测到。因此，约10%的肝脏硒与谷胱甘肽过氧化物酶活性无关。五个膳食组分别以亚硒酸钠的形式补充了0、0.05、0.5、5.0和10 ppm的硒。高硒饮食组观察到药物代谢酶有一些变化。非硒谷胱甘肽活性以及用依他尼酸测定的谷胱甘肽转移酶增加，这些被解释为硒中毒的早期迹象。含0.01 ppm硒且无额外补充硒的饮食产生了先前报道的多种肝脏酶调节作用。与补充0.5 ppm硒的对照组相比，以这种极低硒饮食饲养的动物肝脏中谷胱甘肽、α-生育酚、钙、镁、铁、锌、铜和锰的含量正常。然而，观察到微粒体脂肪酸模式有显著变化，而两个膳食组之间的总磷脂含量以及膜流动性没有差异。
