Postlind H
Department of Pharmaceutical Biochemistry, University of Uppsala, Sweden.
Biochem Biophys Res Commun. 1990 Apr 16;168(1):261-6. doi: 10.1016/0006-291x(90)91702-t.
The cytochromes P-450 in pig kidney mitochondria catalyzing 1 alpha-, 24- and 26-hydroxylations of 25-hydroxyvitamin D3 have been separated. The cytochrome P-450 fractions required NADPH, mitochondrial ferredoxin and ferredoxin reductase for catalytic activity. The present report demonstrates that different forms of cytochrome P-450 are involved in 1 alpha-, 24- and 26-hydroxylations of 25-hydroxyvitamin D3 and provides a basis for further purification and characterization of these enzymes.
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