猪肾线粒体中细胞色素P - 450形成26 - 羟基胆固醇的证据。
Evidence for the formation of 26-hydroxycholesterol by cytochrome P-450 in pig kidney mitochondria.
作者信息
Postlind H, Wikvall K
机构信息
Department of Pharmaceutical Biochemistry, University of Uppsala, Sweden.
出版信息
Biochem Biophys Res Commun. 1989 Mar 31;159(3):1135-40. doi: 10.1016/0006-291x(89)92227-4.
Pig kidney mitochondria were found to catalyze the formation of 26-hydroxycholesterol, an inhibitor of cholesterol biosynthesis. The cholesterol 26-hydroxylase was purified 600-fold. It was present in a mitochondrial enzyme fraction enriched in cytochrome P-450. The cytochrome P-450 fraction required NADPH, mitochondrial ferredoxin and ferredoxin reductase for 26-hydroxylase activity. The mitochondria and the purified 26-hydroxylase preparation also catalyzed 26-hydroxylation of 5 beta-cholestane-3 alpha, 7 alpha, 12 alpha-triol, and intermediate in cholic acid biosynthesis, and of 25-hydroxyvitamin D3. The role of extra-hepatic formation of 26-hydroxycholesterol is discussed.
猪肾线粒体被发现可催化胆固醇生物合成抑制剂26-羟基胆固醇的形成。胆固醇26-羟化酶被纯化了600倍。它存在于富含细胞色素P-450的线粒体酶组分中。细胞色素P-450组分需要NADPH、线粒体铁氧还蛋白和铁氧还蛋白还原酶来实现26-羟化酶活性。线粒体和纯化的26-羟化酶制剂还催化了胆酸生物合成中间体5β-胆甾烷-3α,7α,12α-三醇以及25-羟基维生素D3的26-羟化反应。文中讨论了肝外形成26-羟基胆固醇的作用。
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