Research Center for Molecular Biology, Institutes of Life and Health Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, PR China.
Bioresour Technol. 2013 Feb;130:8-15. doi: 10.1016/j.biortech.2012.11.143. Epub 2012 Dec 8.
Solvent extraction of steam exploded lignocellulosic biomass may be a potential way to obtain antioxidative extracts and to enhance the enzymatic convertibility of the solid residue. Boiling solvent extraction (BSE) showed higher solid and phenolic yields than room temperature extraction. Solubilities of phenolics and sugars were higher in anhydrous ethanol (AE) and deionized water (DW) than in ethyl acetate under each individual extraction condition. The antioxidant activities of the AE and DW extract obtained under BSE were better than those of 10mM vitamin C. Conversion of the solid fractions into reducing sugar using Celluclast 1.5L and Novozym 188 after AE and DW extraction was 95.13% and 92.97%, respectively, higher than that obtained with SESB (88.95%).
汽爆木质纤维素生物质的溶剂萃取可能是获得抗氧化提取物并提高固体残渣酶可转化性的一种潜在方法。沸腾溶剂萃取（BSE）显示出比室温萃取更高的固体和酚类产率。在每种单独的萃取条件下，无水乙醇（AE）和去离子水（DW）中酚类和糖的溶解度均高于乙酸乙酯。BSE 下获得的 AE 和 DW 提取物的抗氧化活性优于 10mM 维生素 C。使用 Celluclast 1.5L 和 Novozym 188 将 AE 和 DW 萃取后的固体部分转化为还原糖的转化率分别为 95.13%和 92.97%，高于 SESB（88.95%）。
