Carbohydrate Laboratory, National Food Research Institute, National Agriculture and Food Research Organization, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan.
Bioresour Technol. 2012 Jul;116:529-32. doi: 10.1016/j.biortech.2012.04.004. Epub 2012 Apr 10.
The RT-CaCCO process for enzymatic saccharification was applied to readily-milled fractions of wet sugarcane bagasse. Wet bagasse immediately after juice extraction was crushed with shark-mill blades to prepare two fractions referred to as readily-milled (RF) and hardly-milled fraction (HF). Monosaccharide recoveries from RFs via the RT-CaCCO process were 1.03-1.21 times higher than those from HFs. Moreover, when the wet weight ratio of RF/HF was adjusted to 2/8, the hexose recovery from RF was 90.9%, which was 1.3 times higher than that of the wet bagasse before fractionation. The results show that this process can be used for efficient monosaccharide recovery from RF of wet bagasse. In addition, the process can be adapted to more fibrous HF for multiple uses such as fuel for boilers and fibers for particleboards.
RT-CaCCO 酶解糖化工艺应用于湿式甘蔗渣易磨碎部分。在榨汁后立即用鲨鱼形刀片将湿甘蔗渣粉碎,制备两种易磨碎部分(RF)和难磨碎部分(HF)。通过 RT-CaCCO 工艺从 RF 中回收的单糖回收率比从 HF 中回收的单糖回收率高 1.03-1.21 倍。此外,当 RF/HF 的湿重比调整为 2/8 时,RF 中的己糖回收率为 90.9%,比分馏前的湿甘蔗渣高 1.3 倍。结果表明,该工艺可用于从湿甘蔗渣的 RF 中高效回收单糖。此外,该工艺可适用于更多的纤维 HF,用于锅炉燃料和刨花板纤维等多种用途。