Centro de Ciencias Genómicas, UNAM, Cuernavaca, Morelos CP 62210, Mexico.
Syst Appl Microbiol. 2013 Feb;36(1):33-8. doi: 10.1016/j.syapm.2012.10.006. Epub 2012 Dec 29.
Los Tuxtlas is the northernmost rain forest in North America and is rich in Bradyrhizobium with an unprecedented number of novel lineages. ITS sequence analysis of legumes in polycultures from Los Tuxtlas led to the identification of Phaseolus lunatus and Vigna unguiculata in addition to Phaseolus vulgaris as legumes associated with maize in crops. Bacterial diversity of isolates from nitrogen-fixing nodules of P. lunatus and V. unguiculata was revealed using ERIC-PCR and PCR-RFLP of rpoB genes, and sequencing of recA, nodZ and nifH genes. P. lunatus and V. unguiculata nodule bacteria corresponded to bradyrhizobia closely related to certain native bradyrhizobia from the Los Tuxtlas forest and novel groups were found. This is the first report of nodule bacteria from P. lunatus in its Mesoamerican site of origin and domestication.
洛斯图尔格斯是北美洲最北部的热带雨林，富含豆科根瘤菌，拥有空前数量的新谱系。对来自洛斯图尔格斯的混作种植中的豆科植物的 ITS 序列分析，除了与玉米相关的普通菜豆之外，还鉴定出了利马豆和豇豆。利用 ERIC-PCR 和 rpoB 基因的 PCR-RFLP 以及 recA、nodZ 和 nifH 基因的测序，揭示了来自 P. lunatus 和 V. unguiculata 固氮根瘤的分离物的细菌多样性。P. lunatus 和 V. unguiculata 根瘤细菌与某些来自洛斯图尔格斯森林的本土慢生根瘤菌密切相关，并且发现了新的菌群。这是在中美洲起源和驯化地首次报道的 P. lunatus 根瘤菌。
