溃疡性结肠炎的新范式：调节性 T 细胞是通过免疫失衡诱导/加重 UC 的关键因素。

A new paradigm in ulcerative colitis: regulatory T cells are key factor which induces/exacerbates UC through an immune imbalance.

机构信息

Centre for Gastroenterology and Inflammatory Bowel Disease Research, Hamamatsu South Hospital, Hamamatsu, Japan.

出版信息

Mol Immunol. 2013 Jun;54(2):173-80. doi: 10.1016/j.molimm.2012.11.015. Epub 2012 Dec 29.

DOI:10.1016/j.molimm.2012.11.015

Abstract

Leukocytapheresis (LCAP) appears to remove or inactivate inflammatory cells and to reset immunological responses, resulting to cure responders of ulcerative colitis (UC). The changes of T cell subsets were investigated in UC patients treated with LCAP. Levels of T cell subsets in peripheral blood before and after LCAP were analysed by flow cytometric analysis. Of 20 UC patients, 13 (65%) achieved remission and 2 (10%) showed the improvement of UC symptoms. Ratios of some T cell subtypes such as regulatory T (Treg) cells and memory T cells to CD4(+) T cells changed significantly only in responders. Especially, ratio of resting Treg/CD4(+) T cells was significantly increased after the first LCAP session, and then one of activated Treg/CD4(+) T cells was increased after 2 week. This may lead to the development of a new UC paradigm in which an imbalance in Treg cell subsets triggers the onset and/or exacerbation of UC.

摘要

白细胞吸附疗法（LCAP）似乎可以清除或失活炎症细胞，并重置免疫反应，从而使溃疡性结肠炎（UC）的应答者得到治愈。本研究旨在通过流式细胞术分析探讨白细胞吸附疗法治疗后 UC 患者 T 细胞亚群的变化。通过流式细胞术分析，比较治疗前后外周血 T 细胞亚群的水平。在 20 例 UC 患者中，13 例（65%）达到缓解，2 例（10%）UC 症状改善。仅在应答者中，一些 T 细胞亚群（如调节性 T 细胞[Treg]和记忆 T 细胞）与 CD4+T 细胞的比例发生了显著变化。特别是，在第一次 LCAP 治疗后，静止 Treg/CD4+T 细胞的比例显著增加，然后在 2 周后，活化 Treg/CD4+T 细胞的比例增加。这可能导致一种新的 UC 发病模式的出现，其中 Treg 细胞亚群的失衡触发 UC 的发生和/或加重。