Yokoyama Yoko, Fukunaga Ken, Fukuda Yoshihiro, Tozawa Katsuyuki, Kamikozuru Koji, Ohnishi Kunio, Kusaka Takeshi, Kosaka Tadashi, Hida Nobuyuki, Ohda Yoshio, Miwa Hiroto, Matsumoto Takayuki
Division of Lower Gastroenterology, Department of Internal Medicine, Hyogo College of Medicine, Hyogo, Japan.
Dig Dis Sci. 2007 Oct;52(10):2725-31. doi: 10.1007/s10620-006-9560-z. Epub 2007 Apr 3.
Low-CD25(High+)CD4(+), a subset of regulatory CD25(+)CD4(+) T cells and high-inflammatory CD28(-)CD4(+) T cells can exacerbate ulcerative colitis (UC). This study sought to investigate the frequency of CD25(High+)CD4(+) and CD28(-)CD4(+) T cells in patients with UC and the changes in these cells during Adacolumn granulocyte and monocyte adsorption apheresis (GMA). Subjects were 12 patients with active UC, 11 with quiescent UC, and 14 healthy volunteers (HVs). The mean clinical activity index was 15.7 +/- 2.2 in active UC and 4.5 +/- 1.1 in quiescent UC. Peripheral blood samples were stained with CD4, CD25, and CD28 antibodies for flow cytometry. Patients with active UC received GMA and blood samples were examined before and after the first GMA session. Patients with active UC (P < 0.04) or quiescent UC (P < 0.02) had a higher percentage of CD28(-)D4(+)T cells compared with HVs, while the percentage of CD28(+)CD4(+) T cells was lower in both UC groups compared with HVs (P = 0.03 and P < 0.02). Patients with active UC had a lower percentage of CD25(High+)CD4(+)T cells compared with quiescent UC patients (P < 0.001). A significant increase in CD25(High+)CD4(+) T cells was associated with GMA (P < 0.03). Low CD25(High+)CD4(+) and high CD28(-)CD4(+) are prominent features in UC. The increase in CD25(High+)CD4(+) T cells induced by GMA should contribute to improved immune function. Additional studies are warranted, since a low frequency of CD25(High+)CD4(+) (-) and a high frequency of CD28(-)CD4(+) (-) expressing T cells might be a predictor of clinical response to GMA.
低CD25（高+）CD4（+），即调节性CD25（+）CD4（+）T细胞的一个亚群以及高炎症性CD28（-）CD4（+）T细胞，可加重溃疡性结肠炎（UC）。本研究旨在调查UC患者中CD25（高+）CD4（+）和CD28（-）CD4（+）T细胞的频率以及在阿达柱粒细胞和单核细胞吸附去除术（GMA）过程中这些细胞的变化。研究对象为12例活动期UC患者、11例静止期UC患者和14名健康志愿者（HV）。活动期UC患者的平均临床活动指数为15.7±2.2，静止期UC患者为4.5±1.1。外周血样本用CD4、CD25和CD28抗体染色以进行流式细胞术检测。活动期UC患者接受GMA治疗，并在首次GMA治疗前后检查血样。活动期UC患者（P<0.04）或静止期UC患者（P<0.02）的CD28（-）D4（+）T细胞百分比相较于HV更高，而两个UC组的CD28（+）CD4（+）T细胞百分比相较于HV更低（P=0.03和P<0.02）。活动期UC患者的CD25（高+）CD4（+）T细胞百分比相较于静止期UC患者更低（P<0.001）。CD25（高+）CD4（+）T细胞的显著增加与GMA相关（P<0.03）。低CD25（高+）CD4（+）和高CD28（-）CD4（+）是UC的突出特征。GMA诱导的CD25（高+）CD4（+）T细胞增加应有助于改善免疫功能。鉴于低频率的CD25（高+）CD4（+）（-）和高频率的CD28（-）CD4（+）（-）表达T细胞可能是对GMA临床反应的一个预测指标，因此有必要进行更多研究。
