Belágyi J, Gróf P, Pallai G, Tigyi J
Acta Biochim Biophys Acad Sci Hung. 1979;14(3):183-8.
Nitroxide radical active esters were used to label the muscle protein actin and to study the orientation dependence of the EPR spectra of the labelled protein. It is concluded that the labels are located at least at two different sites of the protein with strong polar environment and different mobilities. The EPR spectrum of the strongly immobilized labels exhibits orientation dependence, the N--O bond axis of the spin labels is nearly perpendicular to the long axis of the F-actin threads, and the labels undergo a rapid rotational motion about an axis directed perpendicular to the filament axis. The application of the nitroxide radical active esters may be useful in the study of ordered systems as muscle or muscle protein systems.
氮氧化物自由基活性酯被用于标记肌肉蛋白肌动蛋白，并研究标记蛋白的电子顺磁共振（EPR）谱的取向依赖性。得出的结论是，这些标记至少位于蛋白质的两个具有强极性环境和不同迁移率的不同位点。强固定化标记的EPR谱表现出取向依赖性，自旋标记的N—O键轴几乎垂直于F-肌动蛋白丝的长轴，并且标记围绕垂直于细丝轴的方向的轴进行快速旋转运动。氮氧化物自由基活性酯的应用可能有助于研究如肌肉或肌肉蛋白质系统等有序系统。
