Belágyi J
Acta Biochim Biophys Acad Sci Hung. 1975;10(3):233-42.
Glycerinated muscle fibres labelled with a set of five-membered maleimide spin labels revealed a complex type epr spectra, which showed a reduced rate of anisotropic motion. The spectra can be interpreted in terms of the ratio of the two down-field peaks and of the hyperfine coupling constant. The spectra clearly indicated that the preferred orientation of the 2peta molecular orbital of the odd electron is perpendicular rather than parallel to the long axis of the muscle fibres. Increasing chain length between the meleimide ring and pyrrolidine nitroxide ring reduces the orientational anisotropy. Thermal denaturation destroys the orientation dependence of the epr spectra of spin labels. The Mg-ATP induced shortening of the fibres influences the spectral parameters. The fibers which had been contracted showed a modified orientation dependence of epr spectra.
用一组五元马来酰亚胺自旋标记物标记的甘油化肌纤维显示出复杂的电子顺磁共振（epr）光谱类型，其各向异性运动速率降低。该光谱可以根据两个低场峰的比例和超精细耦合常数来解释。光谱清楚地表明，奇数电子的2peta分子轨道的优选取向垂直于而不是平行于肌纤维的长轴。增加马来酰亚胺环和吡咯烷氮氧化物环之间的链长会降低取向各向异性。热变性破坏了自旋标记物epr光谱的取向依赖性。Mg-ATP诱导的纤维缩短会影响光谱参数。已经收缩的纤维显示出epr光谱的取向依赖性发生了改变。
