Haginaka Jun
School of Pharmacy and Pharmaceutical Sciences, Mukogawa Women's University, Nishinomiya, Japan.
Methods Mol Biol. 2013;970:377-92. doi: 10.1007/978-1-62703-263-6_24.
Capillary electrophoresis (CE) methods using proteins as the chiral selectors have been developed for the separation of enantiomeric mixtures. For chiral separations in protein-based CE, two methods were utilized. One is affinity capillary electrochromatography (ACEC), and the other is affinity CE (ACE). This chapter deals with the advantages and disadvantages of ACEC and ACE. Furthermore, enantioseparations utilizing ACEC based on packed α(1)-acid glycoprotein-immobilized silica gels, immobilized avidin to fused silica capillaries and ACE based on penicillin G-acylase dissolved in the running buffer are described in detail.
已开发出使用蛋白质作为手性选择剂的毛细管电泳（CE）方法来分离对映体混合物。对于基于蛋白质的CE中的手性分离，采用了两种方法。一种是亲和毛细管电色谱法（ACEC），另一种是亲和CE（ACE）。本章讨论了ACEC和ACE的优缺点。此外，还详细描述了基于填充有固定化α(1)-酸性糖蛋白的硅胶、固定化抗生物素蛋白到熔融石英毛细管的ACEC以及基于溶解在运行缓冲液中的青霉素G-酰化酶的ACE进行的对映体分离。
