Millot M C
Laboratoire de Recherche sur les Polymères, CNRS-Université Paris 12, UMR 7581, 2 à 8 rue Henri Dunant, Thiais 94320, France.
J Chromatogr B Analyt Technol Biomed Life Sci. 2003 Nov 25;797(1-2):131-59. doi: 10.1016/j.jchromb.2003.08.035.
Proteins display interesting chiral discrimination properties owing to multiple possibilities of intermolecular interactions with chiral compounds. This review deals with proteins which have been used as immobilized chiral selectors for the enantioseparation of drugs in liquid chromatography and capillary electrophoresis. The main procedures allowing the immobilization of proteins onto matrices, such as silica and zirconia particles, membranes and capillaries are first presented. Then the factors affecting the enantioseparation of drugs in liquid chromatography, using various protein-based chiral stationary phases (CSPs), are reviewed and discussed. Last, chiral separations already achieved using immobilized protein selectors in affinity capillary electrochromatography (ACEC) are presented and compared in terms of efficiency, stability and reproducibility.
由于蛋白质与手性化合物之间存在多种分子间相互作用的可能性，因此蛋白质具有有趣的手性识别特性。本综述涉及已被用作固定化手性选择剂的蛋白质，用于在液相色谱和毛细管电泳中对药物进行对映体分离。首先介绍了将蛋白质固定到基质（如硅胶和氧化锆颗粒、膜和毛细管）上的主要方法。然后，综述并讨论了使用各种基于蛋白质的手性固定相（CSP）在液相色谱中影响药物对映体分离的因素。最后，介绍了在亲和毛细管电色谱（ACEC）中使用固定化蛋白质选择剂已经实现的手性分离，并在效率、稳定性和重现性方面进行了比较。
