Kawaguchi S
Department of Microbiology and Immunology, Shimane Medical University, Izumo, Japan.
Cell Immunol. 1990 May;127(2):391-404. doi: 10.1016/0008-8749(90)90141-d.
Low-density lipoproteins from chicken egg yolk (EyLDL), which are reactive with mouse antibodies against bromelain-treated mouse erythrocytes (BrMRBC), were conjugated with fluorescein isothiocyanate (FITC). FITC-EyLDL could bind specifically to mouse anti-phospholipid B cells, which comprised all the BrMRBC-rosette-forming cells and anti-BrMRBC lipopolysaccharide-reactive B cells, C3H/He mice at 12 weeks of age had, approximately, 7 x 10(5) EyLDL-binding cells in the peritoneal cavity, 3 x 10(5) EyLDL-binding cells in the pleural cavity, and 3 x 10(5) EyLDL-binding cells in the spleen. In ontogeny, the numbers of EyLDL-binding cells in the peritoneal cavity expanded greatly by 4 weeks. Other normal strains of mice and C3H/HeJ mice at 12 weeks of age had 4-7 x 10(5) EyLDL-binding cells in the peritoneal cavity; the numbers were large (19 x 10(5] in NZB mice, rather small (2 x 10(5] in MRL/lpr mice, and very small (0.1 x 10(5] in CBA/N mice. In some of various strains of mice at 12 months of age, more than 20% of peritoneal cells were EyLDL-binding cells; in particular, all of five older NZB mice examined had more than 10(7) EyLDL-binding cells in the peritoneal cavity.
来自鸡卵黄的低密度脂蛋白（EyLDL）可与抗菠萝蛋白酶处理的小鼠红细胞（BrMRBC）的小鼠抗体发生反应，将其与异硫氰酸荧光素（FITC）偶联。FITC-EyLDL可特异性结合小鼠抗磷脂B细胞，这些细胞包括所有形成BrMRBC玫瑰花结的细胞以及抗BrMRBC脂多糖反应性B细胞。12周龄的C3H/He小鼠腹腔中约有7×10⁵个EyLDL结合细胞，胸腔中有3×10⁵个EyLDL结合细胞，脾脏中有3×10⁵个EyLDL结合细胞。在个体发育过程中，腹腔中EyLDL结合细胞的数量在4周时大幅增加。其他正常品系的12周龄小鼠和C3H/HeJ小鼠腹腔中有4 - 7×10⁵个EyLDL结合细胞；数量在NZB小鼠中较多（19×10⁵），在MRL/lpr小鼠中较少（2×10⁵），在CBA/N小鼠中非常少（0.1×10⁵）。在一些12月龄的不同品系小鼠中，超过20%的腹腔细胞是EyLDL结合细胞；特别是，所检查的五只老年NZB小鼠腹腔中均有超过10⁷个EyLDL结合细胞。
