Kawaguchi S
Dev Comp Immunol. 1986 Winter;10(1):69-78. doi: 10.1016/0145-305x(86)90045-5.
In lymphoid tissues of mice, there exist LPS-reactive B cells which can differentiate to IgM-secreting plaque-forming cells (IgM-PFC) and PFC secreting antibodies against bromelain-treated mouse erythrocytes (BrMRBC) by LPS activation. In this study, four groups of LPS-reactive B cells in spleen, peritoneal cavity and mesenteric lymph nodes from 2- and 10-week-old mice were compared and enumerated as precursors of IgM-PFC and anti-BrMRBC PFC on days 1 and 2 after LPS activation in quantitative culture conditions. The induction of each of four PFC responses in peritoneal cells was sensitive to LPS and anti-mouse IgM antibodies as much as the induction of the respective PFC response in spleen cells. The ratios of four groups of PFC to each other were different among three tissues and between two ages. These findings support the view that the four groups of LPS-reactive B cells in each tissue are mostly in distinct subpopulations of B cells from each other, and the respective groups of different lymphoid tissues at different ages belong to the same subpopulation.
在小鼠的淋巴组织中，存在脂多糖反应性B细胞，这些细胞可通过脂多糖激活分化为分泌IgM的噬斑形成细胞（IgM-PFC）以及分泌抗菠萝蛋白酶处理的小鼠红细胞（BrMRBC）抗体的PFC。在本研究中，对来自2周龄和10周龄小鼠的脾脏、腹腔和肠系膜淋巴结中的四组脂多糖反应性B细胞进行了比较，并在定量培养条件下，于脂多糖激活后的第1天和第2天，将其作为IgM-PFC和抗BrMRBC PFC的前体进行计数。腹腔细胞中四种PFC反应的诱导对脂多糖和抗小鼠IgM抗体的敏感性与脾细胞中相应PFC反应的诱导相同。三组组织之间以及两个年龄组之间，四组PFC相互之间的比例各不相同。这些发现支持这样一种观点，即每个组织中的四组脂多糖反应性B细胞大多彼此处于不同的B细胞亚群中，并且不同年龄的不同淋巴组织中的相应组属于同一亚群。
