Institute of Hematology and Oncology, Hospital Clínic, IDIBAPS, University of Barcelona, Barcelona, Spain.
Leuk Lymphoma. 2013 Sep;54(9):2016-22. doi: 10.3109/10428194.2012.763123. Epub 2013 Jan 29.
Chronic lymphocytic leukemia (CLL) is frequently associated with autoimmune hemolytic anemia (AIHA). However, the mechanisms governing the association between CLL and AIHA are poorly understood. MicroRNAs (miRNAs) have been associated with different clinico-biological forms of CLL and are also known to play a substantial role in autoimmunity. However, there are no studies correlating miRNA expression with the likelihood that patients with CLL will develop AIHA. In this study, we found that malignant B-cells from patients with CLL subsequently developing AIHA present nine down-regulated (i.e. miR-19a, miR-20a, miR-29c, miR-146b-5p, miR-186, miR-223, miR-324-3p, miR-484 and miR-660) miRNAs. Interestingly, two of these miRNAs (i.e. miR-20a and miR-146b-5p) are involved in autoimmune phenomena, and one (i.e. miR-146b-5p) in both autoimmunity and CLL. Furthermore, we demonstrated that miR-146b-5p modulates CD80, a molecule associated with the B-T-cell synapse and in restoration of the antigen presenting cell capacity of CLL cells.
慢性淋巴细胞白血病（CLL）常伴有自身免疫性溶血性贫血（AIHA）。然而，调控 CLL 与 AIHA 之间关联的机制尚不清楚。微小 RNA（miRNA）与不同临床生物学形式的 CLL 相关，并且已知在自身免疫中发挥重要作用。然而，目前尚无研究将 miRNA 表达与 CLL 患者发生 AIHA 的可能性相关联。在这项研究中，我们发现随后发生 AIHA 的 CLL 患者的恶性 B 细胞存在 9 种下调的 miRNA（即 miR-19a、miR-20a、miR-29c、miR-146b-5p、miR-186、miR-223、miR-324-3p、miR-484 和 miR-660）。有趣的是，其中两种 miRNA（miR-20a 和 miR-146b-5p）参与自身免疫现象，而另一种 miRNA（miR-146b-5p）既参与自身免疫又参与 CLL。此外，我们证明了 miR-146b-5p 调节 CD80，CD80 是与 B-T 细胞突触相关的分子，并恢复了 CLL 细胞的抗原呈递细胞能力。
