Xing Limin, Xu Wenyan, Qu Yingying, Zhao Manjun, Zhu Hongli, Liu Hong, Wang Huaquan, Su Xin, Shao Zonghong
Hematology Department of General Hospital, Tianjin Medical University, Tianjin, 300052, China.
Hematology department of the 1st People's Hospital of Jining, Shandong, 272011, China.
Int J Hematol. 2018 Jun;107(6):666-672. doi: 10.1007/s12185-018-2429-z. Epub 2018 Feb 27.
The objective of the study was to study the regulation of B lymphocytes in patients with autoimmune hemolytic anemia (AIHA)/Evans syndrome. From October 2015 to May 2016, 35 patients with AIHA/Evans in the Department of Hematology, Tianjin Medical University General Hospital were enrolled into this study. c-Myb mRNA and miR-150 expression in B lymphocytes were measured using real-time PCR (RT-PCR). Correlation between c-Myb and miR-150 and clinical parameters in patients with AIHA/Evans were analyzed. c-Myb mRNA expression in hemolysis patients was significantly higher than in remission patients and CLL patients, negatively correlated with hemoglobin (Hb) level and complement 3(C3) levels, and positively correlated with total bilirubin (TBIL) concentration and indirect bilirubin (IBIL) concentration. miR-150 expression in hemolysis patients was significantly lower than in patients in remission and CLL patients. miR-150 was negatively correlated with TBIL and IBIL, and positively correlated with Hb, C3. c-Myb mRNA expression levels in hemolytic episode patients were negatively correlated with the expression levels of miR-150. The expression of c-Myb, a regulatory factor of B lymphocytes, is increased in B lymphocytes of AIHA/Evans patients, while miR-150 expression is decreased. c-Myb was negatively correlated with miR-150.
本研究的目的是探讨自身免疫性溶血性贫血（AIHA）/伊文氏综合征患者B淋巴细胞的调控机制。2015年10月至2016年5月，天津医科大学总医院血液科35例AIHA/伊文氏综合征患者纳入本研究。采用实时荧光定量PCR（RT-PCR）检测B淋巴细胞中c-Myb mRNA和miR-150的表达。分析AIHA/伊文氏综合征患者c-Myb和miR-150与临床参数之间的相关性。溶血患者c-Myb mRNA表达显著高于缓解期患者和慢性淋巴细胞白血病（CLL）患者，与血红蛋白（Hb）水平和补体3（C3）水平呈负相关，与总胆红素（TBIL）浓度和间接胆红素（IBIL）浓度呈正相关。溶血患者miR-150表达显著低于缓解期患者和CLL患者。miR-150与TBIL和IBIL呈负相关，与Hb、C3呈正相关。溶血发作期患者c-Myb mRNA表达水平与miR-150表达水平呈负相关。AIHA/伊文氏综合征患者B淋巴细胞中B淋巴细胞调节因子c-Myb表达增加，而miR-150表达降低。c-Myb与miR-150呈负相关。
