Department of Biotechnology, Mepco Schlenk Engineering College, Sivakasi, India.
Indian J Med Res. 2012 Nov;136(5):822-6.
BACKGROUND & OBJECTIVES: Derivatives of isatin are known to have cytotoxicity against human carcinoma cell lines. This compound therefore, has a potential to be used as a chemotherapeutic agent against cancer. This study was done to investigate the antioxidant and anticancer activities of isatin, extracted from flower of a folklore medicinal plant Couroupita guianensis against human promylocytic leukemia (HL60) cells.
Active fractions demonstrating anticancer and antioxidant activities were isolated from the extracts of shade-dried flowers of C. guianensis by bioassay guided fractionation. The free radical scavenging activity was determined using lipid peroxidation assay. Cytotoxicity against human promylocytic leukemia HL60 cells was determined by MTT assay. Apoptotic activity was analyzed by DNA fragmentation and flowcytometry.
Isatin isolated from the active fraction showed antioxidant activity with the EC(50) value of 72.80 μg/ml. It also exhibited cytotoxicity against human promylocytic leukemia HL60 cells in dose-dependant manner with the CC(50) value of 2.94 μg/ml. The isatin-treated cells underwent apoptosis and DNA fragmentation. Apoptosis was confirmed by the FACS analysis using FITC-annexin V markers.
INTERPRETATION & CONCLUSIONS: Isatin showed antioxidant activity and was cytotoxic to the HL60 cells due to induction of apoptosis. The isatin can be further evaluated to be used as a prophylactic agent to prevent the free radical-induced cancer and as a chemotherapeutic agent to kill the cancer cells.
靛红衍生物已被证实对人类癌细胞系具有细胞毒性。因此，该化合物有可能被用作抗癌化疗药物。本研究旨在探讨从民俗药用植物大花可可树的花中提取的靛红对人早幼粒细胞白血病（HL60）细胞的抗氧化和抗癌活性。
通过生物测定指导的分步分离，从大花可可树阴干花提取物中分离出具有抗癌和抗氧化活性的活性部位。使用脂质过氧化测定法测定自由基清除活性。通过 MTT 测定法测定对人早幼粒细胞白血病 HL60 细胞的细胞毒性。通过 DNA 片段化和流式细胞术分析分析凋亡活性。
从活性部位分离出的靛红具有抗氧化活性，EC（50）值为 72.80μg/ml。它还表现出对人早幼粒细胞白血病 HL60 细胞的剂量依赖性细胞毒性，CC（50）值为 2.94μg/ml。靛红处理的细胞经历凋亡和 DNA 片段化。通过使用 FITC-annexin V 标记物的 FACS 分析确认了凋亡。
靛红显示出抗氧化活性，并通过诱导细胞凋亡对 HL60 细胞具有细胞毒性。可以进一步评估靛红作为预防自由基诱导的癌症的预防剂和作为杀死癌细胞的化疗药物。
