Synthesis, Characterization, and Antibacterial Activity of New Isatin Derivatives.

1H-indol-2,3-dione (isatin) class of biologically active compounds have analgesic, anti-microbial, anti-inflammatory, anti-tubercular, anti-proliferative properties, and is also useful for the treatment of SARS-CoV. Schiff bases containing isatin moiety are known to have broad spectrum of biological activities like anti-viral, anti-tubercular, anti-fungal, and anti-bacterial. In this work, several Schiff base derivatives have been synthesized using two methods (synthetic and microwave) by reacting isatin with -phenylenediamine. The synthesized compounds were structurally characterized and their antimicrobial activity was tested against Gram-negative and Gram-positive bacteria using the inhibition zone method. Several newly synthesized isatin derivatives were found effective as antimicrobial agents and showed good potency (compounds , , , , ). Compound displayed higher antimicrobial activity than standard drug (Amoxicillin) against at higher concentration (16 μg/mL) and against at lower concentration (1 μg/mL).