Costa Danielle Cristina Machado, Azevedo Mariana Maria Barros de, Silva Davi Oliveira E, Romanos Maria Teresa Villela, Souto-Padrón Thais Cristina Baeta Soares, Alviano Celuta Sales, Alviano Daniela Sales
a Institute of Microbiology Paulo de Góes , Federal University of Rio de Janeiro (IMPG-UFRJ), CCS, Ilha do Fundão , Rio de Janeiro , Brazil.
b Chemistry Institute , Federal University of Rio de Janeiro (UFRJ), CT, Ilha do Fundão , Rio de Janeiro , Brazil.
Nat Prod Res. 2017 Sep;31(17):2077-2080. doi: 10.1080/14786419.2016.1272110. Epub 2016 Dec 26.
Couroupita guianensis is known in Brazil as 'Abricó-de-Macaco' and it has some attributes such as: antihypertensive, analgesic and anti-inflammatory activities. This study evaluated the antimicrobial activity of ethanolic extract and fractions of C. guianensis flowers and isolation of bioactive component. These extracts and fractions were subjected to agar diffusion, MIC, TLC and bioautography to bacteria, filamentous fungi and yeasts. Among the fractions of EtOH extract, the DCM fraction was the most active, particularly against Methicillin-resistant Staphylococcus aureus (MRSA) with MIC of 156 μg/mL. The active compound in this fraction was identified as Tryptanthrin, which showed promising antibacterial activity for MRSA showing MIC of 62.5 μg/mL. Ultrastructural analysis of MRSA incubated in the presence of Tryptanthrin by transmission electron microscope showed significant alterations in the cellular structure. Cytotoxicity tests demonstrated that DCM fraction and Tryptanthrin showed low toxicity, which makes it a promising candidate for alternative therapies to control and combat diseases.
圭亚那炮弹树在巴西被称为“猕猴番荔枝”，它具有一些特性，如：抗高血压、镇痛和抗炎活性。本研究评估了圭亚那炮弹树花的乙醇提取物及其馏分的抗菌活性，并对生物活性成分进行了分离。这些提取物和馏分针对细菌、丝状真菌和酵母进行了琼脂扩散、最低抑菌浓度（MIC）、薄层色谱（TLC）和生物自显影分析。在乙醇提取物的馏分中，二氯甲烷馏分活性最强，尤其对耐甲氧西林金黄色葡萄球菌（MRSA）有活性，其MIC为156μg/mL。该馏分中的活性化合物被鉴定为色胺酮，对MRSA显示出有前景的抗菌活性，MIC为62.5μg/mL。通过透射电子显微镜对在色胺酮存在下培养的MRSA进行超微结构分析，结果显示细胞结构有显著改变。细胞毒性试验表明，二氯甲烷馏分和色胺酮显示出低毒性，这使其成为控制和对抗疾病的替代疗法的有前景候选物。
