Department of Bioscience, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan.
J Biosci Bioeng. 2013 May;115(5):481-4. doi: 10.1016/j.jbiosc.2012.11.024. Epub 2013 Jan 1.
Two chemically defined media, CDM-1G and CDM-1X, that use glucose and xylose as carbon sources, respectively, were prepared for Lactococcus lactis strain IO-1. The maximal cell density at 600 nm in CDM-1G exceeded 2. Omission growth experiments indicated that IO-1 is auxotrophic for 2 vitamins and 6 amino acids.
两种化学成分确定的培养基 CDM-1G 和 CDM-1X,分别以葡萄糖和木糖作为碳源,被用于制备乳球菌 IO-1。在 CDM-1G 中,600nm 处的最大细胞密度超过了 2.0。生长实验表明,IO-1 需要 2 种维生素和 6 种氨基酸才能生长。