大鼠离体肾小管中的苯丙氨酸羟化作用。
Phenylalanine hydroxylation in isolated rat kidney tubules.
作者信息
Askin D, Green A K, Dickson A J, Fisher M J
机构信息
Department of Biochemistry, University of Liverpool, England.
出版信息
Int J Biochem. 1990;22(1):107-14. doi: 10.1016/0020-711x(90)90085-h.
PMID:2328817
Abstract
- Phenylalanine hydroxylation has been demonstrated to occur in isolated rat kidney tubules under physiological conditions. 2. The hydroxylation flux response is hyperbolic with apparent Km and Vmax values of ca 85 microM phenylalanine and 49 nmol tyrosine formed/mg dry wt per hr respectively. 3. Hydroxylation in kidney tubules is substantially less sensitive to effectors of cyclic AMP turnover and Ca2+ mobilization than phenylalanine hydroxylation in isolated liver cells.
摘要
- 已证实在生理条件下，苯丙氨酸羟化作用发生于分离出的大鼠肾小管中。2. 羟化通量反应呈双曲线，苯丙氨酸的表观 Km 值约为 85 μM，Vmax 值分别为每小时每毫克干重形成 49 nmol 酪氨酸。3. 与分离的肝细胞中的苯丙氨酸羟化作用相比，肾小管中的羟化作用对环磷酸腺苷周转效应器和 Ca2+ 动员的敏感性要低得多。
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