胰岛素在离体肝细胞中对胰高血糖素依赖性苯丙氨酸羟化调控的调节作用。
The role of insulin in the modulation of glucagon-dependent control of phenylalanine hydroxylation in isolated liver cells.
作者信息
Fisher M J, Dickson A J, Pogson C I
出版信息
Biochem J. 1987 Mar 15;242(3):655-60. doi: 10.1042/bj2420655.
Abstract
The stimulation of phenylalanine hydroxylation in isolated liver cells by sub-maximally effective concentrations of glucagon (less than 0.1 microM) is antagonized by insulin (0.1 nM-0.1 microM). This phenomenon is a consequence of a decrease in the glucagon-stimulated phosphorylation of phenylalanine hydroxylase from liver cells incubated in the presence of insulin. The impact of insulin on the phosphorylation state and activity of the hydroxylase is mimicked by incubation of liver cells in the presence of orthovanadate (10 microM). A series of cyclic AMP and cyclic GMP analogues enhanced phenylalanine hydroxylation: in each case insulin diminished the stimulation of flux. These results are discussed in the light of the characteristics of insulin action on other metabolic processes.
摘要
胰高血糖素（浓度低于0.1微摩尔）以亚最大有效浓度刺激分离的肝细胞中苯丙氨酸羟化作用，而胰岛素（0.1纳摩尔 - 0.1微摩尔）可拮抗这种刺激。这种现象是由于在胰岛素存在下孵育的肝细胞中，胰高血糖素刺激的苯丙氨酸羟化酶磷酸化减少所致。在原钒酸盐（10微摩尔）存在下孵育肝细胞可模拟胰岛素对羟化酶磷酸化状态和活性的影响。一系列环磷酸腺苷和环磷酸鸟苷类似物可增强苯丙氨酸羟化作用：在每种情况下，胰岛素都会减弱通量的刺激。根据胰岛素对其他代谢过程的作用特点对这些结果进行了讨论。
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