Department of Biomolecular Science, Gifu University, Yanagido 1-1, Gifu 501-1193, Japan.
Biotechnol Lett. 2013 May;35(5):685-8. doi: 10.1007/s10529-012-1131-0. Epub 2013 Jan 4.
The nitrilase gene of Rhodococcus rhodochrous J1 was expressed in Escherichia coli using the expression vector, pKK223-3. The recombinant E. coli JM109 cells hydrolyzed enantioselectively 2-methyl-2-propylmalononitrile to form (S)-2-cyano-2-methylpentanoic acid (CMPA) with 96 % e.e. Under optimized conditions, 80 g (S)-CMPA l(-1) was produced with a molar yield of 97 % at 30 °C after a 24 h without any by-products.
罗得西亚红红球菌 J1 的腈酶基因在表达载体 pKK223-3 的作用下在大肠杆菌中表达。重组大肠杆菌 JM109 细胞对 2-甲基-2-丙基丙二腈进行对映选择性水解，生成（S）-2-氰基-2-甲基戊酸（CMPA），ee 值为 96%。在优化条件下，30°C 下反应 24 小时后，无需任何副产物，（S）-CMPA 的产量为 80g/L，摩尔收率为 97%。
